{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Jessica Brothwood"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA from single cells"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14891"],"description":["Single cell RNA sequencing data of iPSC derived motor neuron and microglia co-cultures. Cell types include ALS patient harbouring C9ORF72 hexanucleotide repeat expansion (CS29iALS-C9n1), homozygous C9ORF72 knockout (CS29iALS-C9n1.ISOT2RB4 C3.6) and control (CS29iALS-C9n1.ISOT2RB4). All cell types were sequenced after stimulation with E. Coli derived lipopolysaccharides and without stimulation."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Performed according to HT v3.1","Library Construction - Performed according to HT v3.1","Sample Collection - Unstimulated or LPS-primed co-cultures were dissociated using Accutase™ and resuspended in DMEM/F-12 supplemented with 0.04% BSA and 10 µM Y-27632 (72304, StemCell Technologies). Cells were further dissociated by gentle pipetting and filtered through a 35 µm cell strainer (100-0087, StemCell Technologies) before suspension in PBS(-/-) supplemented with 0.04% BSA and 10 µM Y-27632. For each sample, approximately 30,000 cells were pooled from three independent differentiations.","Sequencing - Paired end sequencing was performed using the Illumina NovaSeq6000 platform at 25,000 read depth."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Yujing Gao","Jessica Brothwood"],"additional_accession":[]},"is_claimable":false,"name":"scRNA-seq of motor neuron and microglia co-cultures derived from C9ORF72 HRE iPSCs (ALS patient), C9ORF72 KO iPSCs and control iPSCs in unstimulated and LPS stimulated conditions","description":"Single cell RNA sequencing data of iPSC derived motor neuron and microglia co-cultures. Cell types include ALS patient harbouring C9ORF72 hexanucleotide repeat expansion (CS29iALS-C9n1), homozygous C9ORF72 knockout (CS29iALS-C9n1.ISOT2RB4 C3.6) and control (CS29iALS-C9n1.ISOT2RB4). All cell types were sequenced after stimulation with E. Coli derived lipopolysaccharides and without stimulation.","dates":{"release":"2025-09-05T00:00:00Z","modification":"2025-09-05T09:21:29.682Z","creation":"2025-02-28T14:51:37.041Z"},"accession":"E-MTAB-14891","cross_references":{"ENA":["ERP169768"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0004184"]}}