biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsJessica BrothwoodIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14891Single cell RNA sequencing data of iPSC derived motor neuron and microglia co-cultures. Cell types include ALS patient harbouring C9ORF72 hexanucleotide repeat expansion (CS29iALS-C9n1), homozygous C9ORF72 knockout (CS29iALS-C9n1.ISOT2RB4 C3.6) and control (CS29iALS-C9n1.ISOT2RB4). All cell types were sequenced after stimulation with E. Coli derived lipopolysaccharides and without stimulation.biostudies-arrayexpressNucleic Acid Extraction - Performed according to HT v3.1Library Construction - Performed according to HT v3.1Sample Collection - Unstimulated or LPS-primed co-cultures were dissociated using Accutase™ and resuspended in DMEM/F-12 supplemented with 0.04% BSA and 10 µM Y-27632 (72304, StemCell Technologies). Cells were further dissociated by gentle pipetting and filtered through a 35 µm cell strainer (100-0087, StemCell Technologies) before suspension in PBS(-/-) supplemented with 0.04% BSA and 10 µM Y-27632. For each sample, approximately 30,000 cells were pooled from three independent differentiations.Sequencing - Paired end sequencing was performed using the Illumina NovaSeq6000 platform at 25,000 read depth.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesYujing GaoJessica BrothwoodfalsescRNA-seq of motor neuron and microglia co-cultures derived from C9ORF72 HRE iPSCs (ALS patient), C9ORF72 KO iPSCs and control iPSCs in unstimulated and LPS stimulated conditionsSingle cell RNA sequencing data of iPSC derived motor neuron and microglia co-cultures. Cell types include ALS patient harbouring C9ORF72 hexanucleotide repeat expansion (CS29iALS-C9n1), homozygous C9ORF72 knockout (CS29iALS-C9n1.ISOT2RB4 C3.6) and control (CS29iALS-C9n1.ISOT2RB4). All cell types were sequenced after stimulation with E. Coli derived lipopolysaccharides and without stimulation.2025-09-05T00:00:00Z2025-09-05T09:21:29.682Z2025-02-28T14:51:37.041ZE-MTAB-14891ERP169768EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184