biostudies-arrayexpressAnna CollinHordeum vulgare3DRNAseqCallistohttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14900ABA INSENSITIVE5 (HvABI5) is a transcription factor regulating barley response to drought stress. We conducted RNA-seq for gain-of-function mutant in HvABI5 gene, Hvabi5.d. Comparative transcriptome analysis of RNA from the second leaf below the flag leaf revealed activation of genes involved in stress response and jasmonic acid biosynthesis in Hvabi5.d when drought was applied at heading. In contrast, florigen gene, Flowering Locus T (HvFT), and auxin-related genes showed decreased expression patterns. Additionally, in this study, we measured stomatal conductance and photosynthesis performance under drought applied at heading stage.biostudies-arrayexpressSample Collection - Tissue was collected from the middle part of the second leaf below the flag leaf in four biological replications. Each biological replication contained a fragment of leave from three independent plants.Sequencing - Transcriptome deep sequencing was performed using short-read Illumina sequencing.Library Construction - True Seq libraries were constructed for each sample. PE 150.Sample Treatment - PlantArray lysimeter allows for strict monitoring of the water regimen and accurate drought application based on regular weight measurements and tracking of selected physiological parameters (Appiah et al. 2023). On the 99th day of growth (heading time, growth stage 50-59, Zadoks et al. 1974) 5 of the 9 plants of each genotype growing on a lysimeter were subjected to drought stress. Drought was applied for 15 days. After drought stress (flowering, growth stage 60-69, Zadoks et al. 1974) tissue collection for RNA-seq was performed.Nucleic Acid Extraction - RNA was extracted using a mirVana RNA isolation kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.Growth Protocol - Seed were germinated on Petri dishes at 28°C in a growth chamber. After 5 days seedlings were transplanted to seedling pots and grown at 25°C. On the 20th day of growth plants were placed in 3L pots filled with a mixture of coarse sand with loam (1:3) and maintained under optimal water supply in greenhouse (22/16°C day/night, photoperiod 16/8h). 9 selected plants of each genotype were transferred to a PlantArray intelligent screening system (Plant-DiTech) on the 85th day of growth.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSequence Alignment - RNA-seq reads were aligned to the barley reference transcriptome BaRTv2.18 (Coulter et al. 2022) employing the kallisto tool for accurate transcript quantification (Patro et al., 2017).Data Transformation - The 3D RNA-seq App implemented tools were used for the normalization process.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHordeum vulgareAnna CollinfalseHvABI5 is an important ABA-dependent regulator of drought stress response at heading time in barleyABA INSENSITIVE5 (HvABI5) is a transcription factor regulating barley response to drought stress. We conducted RNA-seq for gain-of-function mutant in HvABI5 gene, Hvabi5.d. Comparative transcriptome analysis of RNA from the second leaf below the flag leaf revealed activation of genes involved in stress response and jasmonic acid biosynthesis in Hvabi5.d when drought was applied at heading. In contrast, florigen gene, Flowering Locus T (HvFT), and auxin-related genes showed decreased expression patterns. Additionally, in this study, we measured stomatal conductance and photosynthesis performance under drought applied at heading stage.2025-08-01T00:00:00Z2025-03-04T08:49:52.575Z2025-03-04T08:49:52.575ZE-MTAB-14900ERP169853E-MTAB-14895EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969