{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Plasmodium falciparum"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14913"],"description":["Sequencing of the genome of Plasmodium falciparum Dd2 lab strain."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - DNA isolation was perform using Qigan DNeasy blood and tissues kit.","Sequencing - The library was run on Illumina NextSeq2000, 2x100 and 20 M reads.","Sample Collection - P. falciparum Dd2 cell culture was performed in Complete media composed of RPMI-1640 with Glutamine and 25 mM HEPES (Gibco), supplemented with100 μM hypoxanthine (from c.c.pro), and gentamicin (Sigma-Aldrich) and supplementation of 10% human serum. Parasites were isolated by saponin treatment (0.01%) from 0.5mL of infected red blood cells at ~5% parasitemia.","Library Construction - Library preparation was done with the DNAseq_Library_Prep, TruSeq DNA PCR Free."],"figure_sub":["MINSEQE Score","Assays and Data","Processed Data","organisation","MAGE-TAB Files"],"data_protocol":["Data Transformation - samtools was used to convert the alignment to a bam file and sort it.","Sequence Alignment - Reads were aligned using bwa (0.7.12) against the Plasmodium falciparum 3D7 reference (PlasmoDB v65)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 2000"],"study_type":["DNA-seq"],"species":["Plasmodium falciparum"],"additional_accession":["ERP170075"],"pubmed_authors":["Fiona Achcar"]},"is_claimable":false,"name":"Plasmodium falciparum Dd2 sequencing","description":"Sequencing of the genome of Plasmodium falciparum Dd2 lab strain.","dates":{"release":"2026-07-21T00:00:00Z","modification":"2026-07-21T09:02:35.887Z","creation":"2025-03-07T16:10:33.505Z"},"accession":"E-MTAB-14913","cross_references":{"ENA":["ERP170075"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0004184"]}}