biostudies-arrayexpressPlasmodium falciparumhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14913Sequencing of the genome of Plasmodium falciparum Dd2 lab strain.biostudies-arrayexpressNucleic Acid Extraction - DNA isolation was perform using Qigan DNeasy blood and tissues kit.Sequencing - The library was run on Illumina NextSeq2000, 2x100 and 20 M reads.Sample Collection - P. falciparum Dd2 cell culture was performed in Complete media composed of RPMI-1640 with Glutamine and 25 mM HEPES (Gibco), supplemented with100 μM hypoxanthine (from c.c.pro), and gentamicin (Sigma-Aldrich) and supplementation of 10% human serum. Parasites were isolated by saponin treatment (0.01%) from 0.5mL of infected red blood cells at ~5% parasitemia.Library Construction - Library preparation was done with the DNAseq_Library_Prep, TruSeq DNA PCR Free.MINSEQE ScoreAssays and DataProcessed DataorganisationMAGE-TAB FilesData Transformation - samtools was used to convert the alignment to a bam file and sort it.Sequence Alignment - Reads were aligned using bwa (0.7.12) against the Plasmodium falciparum 3D7 reference (PlasmoDB v65).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000DNA-seqPlasmodium falciparumERP170075Fiona AchcarfalsePlasmodium falciparum Dd2 sequencingSequencing of the genome of Plasmodium falciparum Dd2 lab strain.2026-07-21T00:00:00Z2026-07-21T09:02:35.887Z2025-03-07T16:10:33.505ZE-MTAB-14913ERP170075EFO_0002944EFO_0004170EFO_0002693EFO_0004917EFO_0005518EFO_0003816EFO_0004184