{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Daniel Eriksson"],"study_type":["proteomic profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14920"],"description":["Paraneoplastic pemphigus is an autoimmune mucocutaneous blistering disease. It is caused by an autoimmune reaction in patients with a malignant neoplasms. We here used proteome arrays to perform a comprehensive study of autoimmune targets in paraneoplastic pemphigus, anti-p200 pemphigoid (Ap200P), and healthy controls. Solid phase protein arrays featuring more than 9000 full-length human proteins (ProtoArray v5.1, PAH05251020, ThermoFisher, Waltham, Mass) were screened with diluted 1:2000 sera from patients affected by paraneoplastic pemphigus, Ap200P, and healthy controls. Formed immunocomplexes were detected with a secondary antibody fluorescent signal (Alexa Fluor 647 goat anti-human IgG, A21445, ThermoFisher). Manufacturer’s protocol was followed using recommended reagents: ProtoArray Blocking Buffer Kit (PA055, ThermoFisher), Dylight 550 goat anti-GST (#DY550011-13-001), Cayman chemicals, Ann Arbor, Mich). Microarrays were scanned using the LuxScan HT24 (BioCapital) scanner."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Hybridization - -","Scaning - Microarrays were scanned using the LuxScan HT24 (BioCapital) scanner.","Labeling - -","Nucleic Acid Extraction - -","Sample Collection - Blood sampling. Serum extraction.","Growth Protocol - -","Sample Treatment - -"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Daniel Eriksson"],"data_protocol":["Data Transformation - -"],"additional_accession":[]},"is_claimable":false,"name":"PNP and ALG1 vs. healthy controls","description":"Paraneoplastic pemphigus is an autoimmune mucocutaneous blistering disease. It is caused by an autoimmune reaction in patients with a malignant neoplasms. We here used proteome arrays to perform a comprehensive study of autoimmune targets in paraneoplastic pemphigus, anti-p200 pemphigoid (Ap200P), and healthy controls. Solid phase protein arrays featuring more than 9000 full-length human proteins (ProtoArray v5.1, PAH05251020, ThermoFisher, Waltham, Mass) were screened with diluted 1:2000 sera from patients affected by paraneoplastic pemphigus, Ap200P, and healthy controls. Formed immunocomplexes were detected with a secondary antibody fluorescent signal (Alexa Fluor 647 goat anti-human IgG, A21445, ThermoFisher). Manufacturer’s protocol was followed using recommended reagents: ProtoArray Blocking Buffer Kit (PA055, ThermoFisher), Dylight 550 goat anti-GST (#DY550011-13-001), Cayman chemicals, Ann Arbor, Mich). Microarrays were scanned using the LuxScan HT24 (BioCapital) scanner.","dates":{"release":"2025-12-07T00:00:00Z","modification":"2025-12-07T12:04:21.562Z","creation":"2025-03-07T17:56:15.139Z"},"accession":"E-MTAB-14920","cross_references":{"EFO":["EFO_0002944","EFO_0003814","EFO_0003813","EFO_0003789","EFO_0002765","EFO_0005518","EFO_0003816","EFO_0003815","EFO_0003969"]}}