biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsDaniel Erikssonproteomic profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14920Paraneoplastic pemphigus is an autoimmune mucocutaneous blistering disease. It is caused by an autoimmune reaction in patients with a malignant neoplasms. We here used proteome arrays to perform a comprehensive study of autoimmune targets in paraneoplastic pemphigus, anti-p200 pemphigoid (Ap200P), and healthy controls. Solid phase protein arrays featuring more than 9000 full-length human proteins (ProtoArray v5.1, PAH05251020, ThermoFisher, Waltham, Mass) were screened with diluted 1:2000 sera from patients affected by paraneoplastic pemphigus, Ap200P, and healthy controls. Formed immunocomplexes were detected with a secondary antibody fluorescent signal (Alexa Fluor 647 goat anti-human IgG, A21445, ThermoFisher). Manufacturer’s protocol was followed using recommended reagents: ProtoArray Blocking Buffer Kit (PA055, ThermoFisher), Dylight 550 goat anti-GST (#DY550011-13-001), Cayman chemicals, Ann Arbor, Mich). Microarrays were scanned using the LuxScan HT24 (BioCapital) scanner.biostudies-arrayexpressHybridization - -Scaning - Microarrays were scanned using the LuxScan HT24 (BioCapital) scanner.Labeling - -Nucleic Acid Extraction - -Sample Collection - Blood sampling. Serum extraction.Growth Protocol - -Sample Treatment - -MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsDaniel ErikssonData Transformation - -falsePNP and ALG1 vs. healthy controlsParaneoplastic pemphigus is an autoimmune mucocutaneous blistering disease. It is caused by an autoimmune reaction in patients with a malignant neoplasms. We here used proteome arrays to perform a comprehensive study of autoimmune targets in paraneoplastic pemphigus, anti-p200 pemphigoid (Ap200P), and healthy controls. Solid phase protein arrays featuring more than 9000 full-length human proteins (ProtoArray v5.1, PAH05251020, ThermoFisher, Waltham, Mass) were screened with diluted 1:2000 sera from patients affected by paraneoplastic pemphigus, Ap200P, and healthy controls. Formed immunocomplexes were detected with a secondary antibody fluorescent signal (Alexa Fluor 647 goat anti-human IgG, A21445, ThermoFisher). Manufacturer’s protocol was followed using recommended reagents: ProtoArray Blocking Buffer Kit (PA055, ThermoFisher), Dylight 550 goat anti-GST (#DY550011-13-001), Cayman chemicals, Ann Arbor, Mich). Microarrays were scanned using the LuxScan HT24 (BioCapital) scanner.2025-12-07T00:00:00Z2025-12-07T12:04:21.562Z2025-03-07T17:56:15.139ZE-MTAB-14920EFO_0002944EFO_0003814EFO_0003813EFO_0003789EFO_0002765EFO_0005518EFO_0003816EFO_0003815EFO_0003969