{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Nares Trakooljul"],"instrument_platform":["Illumina HiSeq 2500"],"study_type":["RNA-seq of coding RNA"],"organism":["Gallus gallus"],"species":["Gallus gallus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14922"],"description":["Phytogenic feed additives offer health benefits, improved overall performance and well-being in poultry production and provide an alternative to in-feed antibiotics. In this experiment, post-peak laying hens were fed for a period of 4 weeks either Control or Supplemental diet, a phytogenic feed additive based with essential oils from thyme (Thymus vulgaris) and star anis (Illicium verum) as well as Quillaja bark (Quillaja Saponaria). RNA sequencing was performed in the pancreas, kidney, uterus and large-white follicles of 10 birds from each dietary groups at the end of the trial."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Indexing libraries were multiplexed and sequenced for 2x71 bp paired-end reads in high-output mode on the Illumina HiSeq 2500.","Nucleic Acid Extraction - RNA was extracted using TRIzol Reagent (Invitrogen, Karlsruhe, Germany), followed by DNase I digestion (Roche, Mannheim, Germany) and finally clean-up using the RNeasy Mini Spin kit (Qiagen).","Sample Collection - At Day 28 of the feeding trial, 10 birds of each dietary group were mechanically stunned and slaughtered by decapitation. Tissue samples were collected from the right kidney, pancreas taken in anatomical proximity to the duodenal loop, uterus mucosa collected 2-5 cm upstream of the cloaca, and 5-10 follicles with an average diameter of 3 to 5 mm (large-white follicles). After dissection, the samples were rinsed with saline solution, snap frozen in liquid nitrogen and stored at -80 °C until use.","Library Construction - Total RNA of 2 µg was used for library preparation with the TruSeq Stranded mRNA Kit according to the manufacturer's recommendation (Illumina, San Diego, CA, USA)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Henry Reyer","Nares Trakooljul","Klaus Wimmers"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq profiling of the kidney, pancreas, follicle, and uterus of laying hens supplemented with phytogenic feed additives","description":"Phytogenic feed additives offer health benefits, improved overall performance and well-being in poultry production and provide an alternative to in-feed antibiotics. In this experiment, post-peak laying hens were fed for a period of 4 weeks either Control or Supplemental diet, a phytogenic feed additive based with essential oils from thyme (Thymus vulgaris) and star anis (Illicium verum) as well as Quillaja bark (Quillaja Saponaria). RNA sequencing was performed in the pancreas, kidney, uterus and large-white follicles of 10 birds from each dietary groups at the end of the trial.","dates":{"release":"2026-01-29T00:00:00Z","modification":"2026-01-29T10:27:11.721Z","creation":"2025-03-07T18:15:40.799Z"},"accession":"E-MTAB-14922","cross_references":{"ENA":["ERP170085"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}