{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Musa sp."],"software":["DESeq2","STAR; samtools"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14954"],"description":["We aim to assess heterosis and regulatory divergence in these Musa genotypes (Musa textilis var Abuab, Musa balbisiana var Pacol, and their backcross hybrid (BC2)) by estimating differential expression between the parents and between the two genotype-specific alleles in the hybrid."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Random fragmentation of cDNA, followed by adapter-ligation, then PCR","Nucleic Acid Extraction - RNA extraction was performed using modified CTAB method with overnight LiCl precipitation.","Sample Collection - 3-month old suckers from the three varieties were randomly selected from the field collection","Sample Treatment - no treatment protocol was implemented as we wanted to test the genotype variations only without additional variable such as treatment","Growth Protocol - Materials were grown under natural field conditions at the Institute of Plant Breeding, UP Los Baños, Laguna. No additional growth treatment was applied as samples were collected after 3-month growth.","Sequencing - Illumina TruSeq RNA Library Prep Kit with Ribo-Zero rRNA reduction"],"figure_sub":["MINSEQE Score","Assays and Data","Processed Data","organisation","MAGE-TAB Files"],"data_protocol":["Sequence Alignment - The RNA-seq reads were aligned against the reference sequences Musa textilis var Abuab and Musa balbisian var DH-PKW using STAR","Data Transformation - Normalization of the data frame was performed using median normalization from DESeq2 package."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["computer","lab equipment","NA","all materials grown under field condition; no hardware involved","Illumina NovaSeq X","bucket and liquid N"],"study_type":["RNA-seq of coding RNA"],"species":["Musa sp."],"additional_accession":["ERP170560"],"pubmed_authors":["NELZO EREFUL"]},"is_claimable":false,"name":"RNA-seq datasets of three Musa genotypes","description":"We aim to assess heterosis and regulatory divergence in these Musa genotypes (Musa textilis var Abuab, Musa balbisiana var Pacol, and their backcross hybrid (BC2)) by estimating differential expression between the parents and between the two genotype-specific alleles in the hybrid.","dates":{"release":"2025-05-12T00:00:00Z","modification":"2026-05-30T16:25:21.049Z","creation":"2025-03-19T17:23:20.141Z"},"accession":"E-MTAB-14954","cross_references":{"ENA":["ERP170560"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184","EFO_0003969"]}}