{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Osamu Hashimoto"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14962"],"description":["HFD was fed to mice in which Activin E  was knocked out to investigate the effect on the liver.Adult mice were fed HFD for 6 weeks, and RNA from the liver was collected to examine whether gene expression in the liver is altered by Activin E knockout."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA was extracted from liver tissue using Isogen II reagent (Nippon Gene, Tokyo, Japan) according to the manufacturer’s protocol.","Sequencing - RNA-seq libraries were sequenced using the Illumina NovaSeq X Plus platform with paired-end 150 bp reads.","Sample Collection - Liver tissue was collected from Activin E KO mice (male). Samples were stored at -80°C until RNA extraction.","Library Construction - mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module. The RNA-seq library was constructed with the NEBNext Ultra II Directional RNA Library Prep Kit following the manufacturer’s instructions, including fragmentation, cDNA synthesis, adapter ligation, and PCR amplification."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Osamu Hashimoto"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of the livers of HFD fed Activin E KO mice","description":"HFD was fed to mice in which Activin E  was knocked out to investigate the effect on the liver.Adult mice were fed HFD for 6 weeks, and RNA from the liver was collected to examine whether gene expression in the liver is altered by Activin E knockout.","dates":{"release":"2026-04-01T00:00:00Z","modification":"2026-04-02T01:04:57.796Z","creation":"2025-03-20T14:13:21.681Z"},"accession":"E-MTAB-14962","cross_references":{"ENA":["ERP170613"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}