{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Zerui Wang"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14966"],"description":["Using isolated and purified round and elongated spermatids from the testes, mRNA sequencing was performed to assess the impact of MEX3D protein deficiency on RNA metabolism in spermiogenesis."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - mRNA was enriched using mRNA Capture Beads, followed by high-temperature fragmentation.","Sequencing - After library screening and purification using Hieff NGS® DNA Selection Beads, the library was amplified and detected using the Illumina Novaseq X Plus platform.","Sample Collection - Round and elongated spermatids were isolated and purified from adult mouse testes.","Library Construction - The first strand of cDNA was synthesized in a reverse transcription mixture, and the second strand was synthesized while performing end repair and adding an A-tail."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Gene-level quantification was performed using featureCounts (v2.0.6), counting paired-end reads (`-p --countReadPairs`) aligned to exon features (`-t exon`) and assigning counts to genes (`-g gene_id`).","Sequence Alignment - For data analysis, paired-end reads were quality-trimmed using fastp (v0.23.4) with default parameters and 16 processing threads. Clean reads were aligned to the mouse reference genome (GRCm39, Ensembl release 112) using STAR (v2.7.11b). The resulting alignments were sorted by coordinates and stored in BAM format."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of total RNA"],"species":["Mus musculus"],"pubmed_authors":["Zerui Wang"],"additional_accession":[]},"is_claimable":false,"name":"RNA Sequencing of Testicular Spermatids in Mex3d Knockout and Wild-Type Mice","description":"Using isolated and purified round and elongated spermatids from the testes, mRNA sequencing was performed to assess the impact of MEX3D protein deficiency on RNA metabolism in spermiogenesis.","dates":{"release":"2026-03-31T00:00:00Z","modification":"2026-03-31T01:03:46.776Z","creation":"2025-03-21T17:08:02.636Z"},"accession":"E-MTAB-14966","cross_references":{"ENA":["ERP170653"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0004184"]}}