{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Qianhui Yu"],"instrument_platform":["Illumina iSeq 100"],"study_type":["DNA-seq"],"organism":["synthetic construct"],"species":["synthetic construct"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14972"],"description":["The STARR-seq plasmid library used for transfection was sequenced to enable input normalization. The sequencing library was generated with the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs) and sequenced on the Illumina iSeq 100"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - The library of the synthetic constructs was sequenced on the Illumina iSeq 100 by the Roche 360 lab core facility","Nucleic Acid Extraction - Endotoxin-free plasmids were purified with ZymoPURE II Plasmid Maxiprep kit (Zymo) and DNA precipitation with 3M Na-Acetate pH 5.2 was performed if necessary to obtain desired concentrations.","Sample Collection - DNA sequences of candidate cis-regulatory elements (CREs) were synthesized using a commercial service (GenScript) and cloned individually into the STARR-seq vector via Gibson assembly.","Library Construction - The sequencing library was generated with the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Qianhui Yu","Stefano Secchia"],"additional_accession":[]},"is_claimable":false,"name":"Sequencing of the STARR-seq transfection plasmid library","description":"The STARR-seq plasmid library used for transfection was sequenced to enable input normalization. The sequencing library was generated with the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs) and sequenced on the Illumina iSeq 100","dates":{"release":"2025-08-08T00:00:00Z","modification":"2025-08-08T09:12:28.129Z","creation":"2025-03-26T18:39:16.132Z"},"accession":"E-MTAB-14972","cross_references":{"ENA":["ERP170867"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0004184"]}}