biostudies-arrayexpressSmiths LueongMus musculusIlluminahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14982The study investigates the role of microbiota on neuroinflammation and stroke. RNA sequencing was performed on mouse brain samples to compare the gene expression patterns in mice who where treated with antibiotics or with sucrose controlbiostudies-arrayexpressSequencing - Total RNA sequencing libraries were sequenced on a NovaSeq 6000 with 100 bp paired end readsLibrary Construction - RNA sequencing libraries were prepared using the KAPA RNA HyperPrep with RiboEraseSample Treatment - Mice were either treated with sucrose or with antibioticsNucleic Acid Extraction - Total RNA was isolated from tissue samples using the RNAeasy minikit, QiagenSample Collection - Brain tissue samples were collected from mice treated with sucrose or antibioticsOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Data was normalized with default tools as implemented in edgeR tool before further downstream analysisSequence Alignment - Sequencing reads were alligned to the mm10 mouse genome build using STAR alignerUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusSmiths LueongfalseGut microbiota induce neutrophil activation that aggravates stroke pathologyThe study investigates the role of microbiota on neuroinflammation and stroke. RNA sequencing was performed on mouse brain samples to compare the gene expression patterns in mice who where treated with antibiotics or with sucrose control2025-12-30T00:00:00Z2025-12-30T02:02:07.743Z2025-03-24T14:22:19.189ZE-MTAB-14982ERP170724EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969