{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Manuela Baquero Perez"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of coding RNA"],"organism":["Saccharomyces cerevisiae"],"species":["Saccharomyces cerevisiae"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14983"],"description":["Gradient-enriched quiescent cells of a 6 days stationary phase culture were refed with rich medium (Yeast Peptone Dextrose 2%) in the presence or absence of RNAPII inhibition by various means (rpb1-1 thermosensitive mutant, thiolutin). Samples were harvested in quiescence and 30 minutes post-refeeding."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Libraries were pair-end 150 sequenced","Library Construction - Samples were processed at Novogene for polyA-enriched library construction","Nucleic Acid Extraction - Total RNA was extracted using Ribopure Yeast RNA (invitrogen AM1926)","Sample Collection - Saccharomyces cerevisiae cells were inoculated at 0.1 OD/mL in rich medium (Yeast Peptone Dextrose 2%, YPD) and grown for 6-7 days into stationary phase in conditions favouring aeration. Then, 25 OD600 nm equivalent of cells were harvested, pelleted at 800g and flash-frozen then stored at -70ºC before processing."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Manuela Baquero Perez","Gertjan Laenen","Angela Taddei"],"additional_accession":[]},"is_claimable":false,"name":"RNAseq in quiescent and refed quiescent cells in Saccharomyces cerevisiae","description":"Gradient-enriched quiescent cells of a 6 days stationary phase culture were refed with rich medium (Yeast Peptone Dextrose 2%) in the presence or absence of RNAPII inhibition by various means (rpb1-1 thermosensitive mutant, thiolutin). Samples were harvested in quiescence and 30 minutes post-refeeding.","dates":{"release":"2025-07-26T00:00:00Z","modification":"2025-07-26T14:10:03.297Z","creation":"2025-03-27T22:35:56.806Z"},"accession":"E-MTAB-14983","cross_references":{"ENA":["ERP170935"],"Biostudies":["E-MTAB-14337"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}