biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsManuela Baquero PerezIllumina NovaSeq XRNA-seq of coding RNASaccharomyces cerevisiaeSaccharomyces cerevisiaehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14983Gradient-enriched quiescent cells of a 6 days stationary phase culture were refed with rich medium (Yeast Peptone Dextrose 2%) in the presence or absence of RNAPII inhibition by various means (rpb1-1 thermosensitive mutant, thiolutin). Samples were harvested in quiescence and 30 minutes post-refeeding.biostudies-arrayexpressSequencing - Libraries were pair-end 150 sequencedLibrary Construction - Samples were processed at Novogene for polyA-enriched library constructionNucleic Acid Extraction - Total RNA was extracted using Ribopure Yeast RNA (invitrogen AM1926)Sample Collection - Saccharomyces cerevisiae cells were inoculated at 0.1 OD/mL in rich medium (Yeast Peptone Dextrose 2%, YPD) and grown for 6-7 days into stationary phase in conditions favouring aeration. Then, 25 OD600 nm equivalent of cells were harvested, pelleted at 800g and flash-frozen then stored at -70ºC before processing.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesManuela Baquero PerezGertjan LaenenAngela TaddeifalseRNAseq in quiescent and refed quiescent cells in Saccharomyces cerevisiaeGradient-enriched quiescent cells of a 6 days stationary phase culture were refed with rich medium (Yeast Peptone Dextrose 2%) in the presence or absence of RNAPII inhibition by various means (rpb1-1 thermosensitive mutant, thiolutin). Samples were harvested in quiescence and 30 minutes post-refeeding.2025-07-26T00:00:00Z2025-07-26T14:10:03.297Z2025-03-27T22:35:56.806ZE-MTAB-14983ERP170935E-MTAB-14337EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184