{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14996"],"description":["Serotonylation can occur on position 5 on histone H3 and result in H3K4me3Q5ser, an epigenetic modification associated with permissive gene expression. To investigate genes directly regulated by H3K4me3Q5ser, we established PANC-1 cells expressing wild-type H3.3 or H3.3Q5A (the mutation of glutamine to alanine at position 5 that blocks H3Q5ser), and then performed RNA-seq."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - The PANC-1 cells were maintained on DMEM supplemented with 10% FBS, 1% penicillin-streptomycin.","Library Construction - RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit","Nucleic Acid Extraction - RNA was extracted using the RNeasy Plus Kit (Qiagen)","Sequencing - Each library was sequenced at 150 bp paired-end on NovaSeq 6000 (Illumina, San Diego, CA, USA)."],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Shanghai Genefund Biotech Co., Ltd Illumina NovaSeq 6000","Illumina NovaSeq 6000"],"study_type":["RNA-seq of total RNA"],"species":["Homo sapiens"],"additional_accession":["ERP171069"],"pubmed_authors":["ShengTan Tan"]},"is_claimable":false,"name":"RNA-seq of H3.3 or H3.3Q5A expressing PANC-1 cells","description":"Serotonylation can occur on position 5 on histone H3 and result in H3K4me3Q5ser, an epigenetic modification associated with permissive gene expression. To investigate genes directly regulated by H3K4me3Q5ser, we established PANC-1 cells expressing wild-type H3.3 or H3.3Q5A (the mutation of glutamine to alanine at position 5 that blocks H3Q5ser), and then performed RNA-seq.","dates":{"release":"2025-05-08T00:00:00Z","modification":"2026-06-06T08:10:31.761Z","creation":"2025-04-02T09:57:10.88Z"},"accession":"E-MTAB-14996","cross_references":{"ENA":["ERP171069"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0004184"]}}