{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14997"],"description":["H3K4me3Q5ser ChIP-seq were performed  in H3.3 or H3.3Q5A expressing PANC-1 cells and TGM2 knockdown PANC-1 cells to investigate genes directly regulated by H3K4me3Q5ser."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - ChIP-seq libraries were constructed using NEBNext Ultra II DNA Library Prep kit","Nucleic Acid Extraction - The cells were fixed with 1% formaldehyde and sonicated for 5 minutes on 4 ℃. Next, chromatin fragments were immunoprecipitated with Protein A/G Magnetic beads coupled with antibodies. The eluates were digested with  RNase A and proteinase K. DNA fragments were recovered using the QIAquick PCR purification kit and used for ChIP-seq library construction.","Sample Collection - The PANC-1 cells were maintained on DMEM supplemented with 10% FBS, 1% penicillin-streptomycin.Collect cells by digesting with trypsin","Sequencing - Each library was sequenced at 150 bp paired-end on NovaSeq 6000 (Illumina, San Diego, CA, USA)."],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Shanghai Genefund Biotech Co., Ltd Illumina NovaSeq 6000","Illumina NovaSeq 6000"],"study_type":["ChIP-seq"],"species":["Homo sapiens"],"additional_accession":["ERP171071"],"pubmed_authors":["ShengTan Tan"]},"is_claimable":false,"name":"H3K4me3Q5ser ChIP-seq of H3.3 or H3.3Q5A expressing PANC-1 cells and TGM2 knockdown PANC-1 cells","description":"H3K4me3Q5ser ChIP-seq were performed  in H3.3 or H3.3Q5A expressing PANC-1 cells and TGM2 knockdown PANC-1 cells to investigate genes directly regulated by H3K4me3Q5ser.","dates":{"release":"2025-05-08T00:00:00Z","modification":"2026-06-06T00:15:28.158Z","creation":"2025-04-02T10:13:44.816Z"},"accession":"E-MTAB-14997","cross_references":{"ENA":["ERP171071"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002692","EFO_0005518","EFO_0004184"]}}