biostudies-arrayexpressHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14997H3K4me3Q5ser ChIP-seq were performed in H3.3 or H3.3Q5A expressing PANC-1 cells and TGM2 knockdown PANC-1 cells to investigate genes directly regulated by H3K4me3Q5ser.biostudies-arrayexpressLibrary Construction - ChIP-seq libraries were constructed using NEBNext Ultra II DNA Library Prep kitNucleic Acid Extraction - The cells were fixed with 1% formaldehyde and sonicated for 5 minutes on 4 ℃. Next, chromatin fragments were immunoprecipitated with Protein A/G Magnetic beads coupled with antibodies. The eluates were digested with RNase A and proteinase K. DNA fragments were recovered using the QIAquick PCR purification kit and used for ChIP-seq library construction.Sample Collection - The PANC-1 cells were maintained on DMEM supplemented with 10% FBS, 1% penicillin-streptomycin.Collect cells by digesting with trypsinSequencing - Each library was sequenced at 150 bp paired-end on NovaSeq 6000 (Illumina, San Diego, CA, USA).MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsShanghai Genefund Biotech Co., Ltd Illumina NovaSeq 6000Illumina NovaSeq 6000ChIP-seqHomo sapiensERP171071ShengTan TanfalseH3K4me3Q5ser ChIP-seq of H3.3 or H3.3Q5A expressing PANC-1 cells and TGM2 knockdown PANC-1 cellsH3K4me3Q5ser ChIP-seq were performed in H3.3 or H3.3Q5A expressing PANC-1 cells and TGM2 knockdown PANC-1 cells to investigate genes directly regulated by H3K4me3Q5ser.2025-05-08T00:00:00Z2026-06-06T00:15:28.158Z2025-04-02T10:13:44.816ZE-MTAB-14997ERP171071EFO_0002944EFO_0004170EFO_0002692EFO_0005518EFO_0004184