{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Margaret Woodhouse"],"organism":["Zea mays"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15007"],"description":["mRNA derived from treated and untreated Fall army worm and Fusarium graminearum on B73, B104, and W22 maize cultivars"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - Temperature controlled greenhouse under normal light conditions.","Sample Collection - A 4-cm stem section centered on the stack node (includes immature stem, stack node, meristem, and immature leaves) was collected 2 days after inoculation.","Sample Collection - Inner-whorl leaf tissue was harvested which included leaf tissue from 2-3 rolled leaves after two days post-fall army worm and control application.","Library Construction - rRNA was depleted using NEB depletion kit and libraries were constructed with NEBNext Ultra II directional library kit by the Iowa State University DNA Sequencing Facility.","Nucleic Acid Extraction - RNA was extracted from 100 mg of ground tissue using the Zymo DIRECT-ZOL RNA Kit (Zymo Cat # R2051).","Sample Treatment - Plants were infested by placing approximately 50 fall armyworm (Spodoptera frugiperda) into the whorl three-week-old plants.","Sequencing - RNA was sequenced on an Illumina S2 NovaSeq6000 flowcell by the Iowa State University DNA Sequencing Facility.","Sample Treatment - Four-week-old plants were inoculated by slitting the stems through the stack nodes and inoculating with 200 uL of Fusarium graminearum spore solution."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - none"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Zea mays"],"pubmed_authors":["Margaret Woodhouse"],"additional_accession":[]},"is_claimable":false,"name":"Fall army worm and Fusarium graminearum stress response RNA-seq studies across three maize genomes","description":"mRNA derived from treated and untreated Fall army worm and Fusarium graminearum on B73, B104, and W22 maize cultivars","dates":{"release":"2026-10-06T00:00:00Z","modification":"2026-10-06T01:00:46.99Z","creation":"2025-04-04T10:20:27.253Z"},"accession":"E-MTAB-15007","cross_references":{"ENA":["ERP171267"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184","EFO_0003969"]}}