biostudies-arrayexpressMargaret WoodhouseZea mayshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15007mRNA derived from treated and untreated Fall army worm and Fusarium graminearum on B73, B104, and W22 maize cultivarsbiostudies-arrayexpressGrowth Protocol - Temperature controlled greenhouse under normal light conditions.Sample Collection - A 4-cm stem section centered on the stack node (includes immature stem, stack node, meristem, and immature leaves) was collected 2 days after inoculation.Sample Collection - Inner-whorl leaf tissue was harvested which included leaf tissue from 2-3 rolled leaves after two days post-fall army worm and control application.Library Construction - rRNA was depleted using NEB depletion kit and libraries were constructed with NEBNext Ultra II directional library kit by the Iowa State University DNA Sequencing Facility.Nucleic Acid Extraction - RNA was extracted from 100 mg of ground tissue using the Zymo DIRECT-ZOL RNA Kit (Zymo Cat # R2051).Sample Treatment - Plants were infested by placing approximately 50 fall armyworm (Spodoptera frugiperda) into the whorl three-week-old plants.Sequencing - RNA was sequenced on an Illumina S2 NovaSeq6000 flowcell by the Iowa State University DNA Sequencing Facility.Sample Treatment - Four-week-old plants were inoculated by slitting the stems through the stack nodes and inoculating with 200 uL of Fusarium graminearum spore solution.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesData Transformation - noneMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAZea maysMargaret WoodhousefalseFall army worm and Fusarium graminearum stress response RNA-seq studies across three maize genomesmRNA derived from treated and untreated Fall army worm and Fusarium graminearum on B73, B104, and W22 maize cultivars2026-10-06T00:00:00Z2026-10-06T01:00:46.99Z2025-04-04T10:20:27.253ZE-MTAB-15007ERP171267EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969