{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["David John"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15019"],"description":["Heart failure with preserved ejection fraction (HFpEF) accounts for half of heart failure cases and is characterised by reduced pericyte coverage. While the contributions of other cardiac cell types to HFpEF are well-studied, the role of pericytes remains less understood. Using murine single-nucleus RNA sequencing to study cardiac pericytes in HFpEF, we identified reduced STAT3 expression as a hallmark of HFpEF pericytes. Mechanistic studies in vitro revealed that STAT3 deletion induces cellular senescence and impairs pericyte adhesion, recapitulating HFpEF-like characteristics. These findings suggest that STAT3 is crucial for maintaining pericyte homeostasis and highlight its reduction as a potential driver of pericyte loss, a defining feature of HFpEF."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Cells were transfected as indicated above and RNA was isolated using RNeasy Plus Mini Kit (74136, Qiagen) according to manufacturer‘s instructions.","Sequencing - . After library quality control by capillary electrophoresis (4200 TapeStation, Agilent), cDNA libraries were sequenced on the Illumina NovaSeq 6000 platform generating 50 bp paired-end reads.","Sample Collection - STAT3 silencing was performed using siRNA (Sigma; sequences: GGAUAACGUCAUUAGCAG, UCUGCUAAUGACGUUAUCC. Concentration, 100µM). hPL-PC were seeded with a density of 77.000 cells/well in a 6-well-plate 24 h before transfection. STAT3 siRNA and control siRNA were used at a final concentration of 50 nM. For each well an independent solution, containing siRNA diluted in 200 µl OptiMEM medium (51985034, Gibco) was prepared. Additionally, 5 µl Lipofectamine RNAiMAX transfection reagent (13778150, ThermoFisher) was diluted in 195 µl OptiMEM. The siRNA diluted in OptiMEM was added to the Lipofectamine mix and incubated for 15 min at room temperature. Cells were washed with 1 ml OptiMEM medium per well. Then, 1.6 ml OptiMEM medium was added per well and the transfection mix (400 µl) was added dropwise. After 4 h incubation the medium containing the transfection reagents was removed, and cells were further cultured in Pericyte Growth Medium 2 (C-28041, PromoCell) as indicated above.","Library Construction - For whole-transcriptome analysis ribosomal RNA (rRNA) was removed from a total amount of 250 ng RNA per sample followed by cDNA sequencing library preparation utilizing the Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (Illumina) according to the manufacturer’s instructions."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Sequence Alignment - The sequencing reads were mapped to GRCH38 by STAR v 2.7.3","Data Transformation - Sequencing reads were quantified using cufflinks and differential expression analysis were performed with cuffdiff. The Values were FPKM normalised."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_title":["STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence"],"pubmed_authors":["David John","Leah Rebecca Vanicek, Ariane Fischer, Mariano Ruz Jurado, Anita Tamiato, Tara Procida-Kowalski, Jochen Wilhelm, Dennis Hecker, Maximilian Merten, Felicitas Escher, Badder Kattih,8, Valentina Puntmann, David John, Marcel H. Schulz, Eike Nagel, Stefanie Dimmeler, and Guillermo Luxán"],"additional_accession":[]},"is_claimable":false,"name":"STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence","description":"Heart failure with preserved ejection fraction (HFpEF) accounts for half of heart failure cases and is characterised by reduced pericyte coverage. While the contributions of other cardiac cell types to HFpEF are well-studied, the role of pericytes remains less understood. Using murine single-nucleus RNA sequencing to study cardiac pericytes in HFpEF, we identified reduced STAT3 expression as a hallmark of HFpEF pericytes. Mechanistic studies in vitro revealed that STAT3 deletion induces cellular senescence and impairs pericyte adhesion, recapitulating HFpEF-like characteristics. These findings suggest that STAT3 is crucial for maintaining pericyte homeostasis and highlight its reduction as a potential driver of pericyte loss, a defining feature of HFpEF.","dates":{"release":"2025-04-25T00:00:00Z","modification":"2025-04-10T11:35:43.34Z","creation":"2025-04-10T11:35:43.34Z"},"accession":"E-MTAB-15019","cross_references":{"ENA":["ERP171469"],"EFO":["EFO_0002944","EFO_0004170","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}