{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Assa Sherman"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15032"],"description":["We perform genomic screen of lncRNAs in hESC to identify growth-modifying genes by the quantification of enrichment or depletion of their KD clones within a pooled library relative to their abundance in the initial time point."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Single ends 74bp","Library Construction - PCR with Illumina adapters and TruSeq indices","Nucleic Acid Extraction - gSYNC DNA extraction kit (Geneaid)","Sample Collection - Harvested using Trypsin"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Not normalized (raw counts)","Sequence Alignment - Used bowtie2 to align to reference gRNAs"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 500"],"study_type":["DNA-seq"],"species":["Homo sapiens"],"pubmed_authors":["Assa Sherman"],"additional_accession":[]},"is_claimable":false,"name":"CRISPRi screen of long non-coding RNAs in human embryonic stem cells","description":"We perform genomic screen of lncRNAs in hESC to identify growth-modifying genes by the quantification of enrichment or depletion of their KD clones within a pooled library relative to their abundance in the initial time point.","dates":{"release":"2025-12-18T00:00:00Z","modification":"2025-12-18T02:01:45.746Z","creation":"2025-04-15T12:48:25.099Z"},"accession":"E-MTAB-15032","cross_references":{"ENA":["ERP171650"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0004184"]}}