biostudies-arrayexpressAssa ShermanHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15032We perform genomic screen of lncRNAs in hESC to identify growth-modifying genes by the quantification of enrichment or depletion of their KD clones within a pooled library relative to their abundance in the initial time point.biostudies-arrayexpressSequencing - Single ends 74bpLibrary Construction - PCR with Illumina adapters and TruSeq indicesNucleic Acid Extraction - gSYNC DNA extraction kit (Geneaid)Sample Collection - Harvested using TrypsinOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Not normalized (raw counts)Sequence Alignment - Used bowtie2 to align to reference gRNAsMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 500DNA-seqHomo sapiensAssa ShermanfalseCRISPRi screen of long non-coding RNAs in human embryonic stem cellsWe perform genomic screen of lncRNAs in hESC to identify growth-modifying genes by the quantification of enrichment or depletion of their KD clones within a pooled library relative to their abundance in the initial time point.2025-12-18T00:00:00Z2025-12-18T02:01:45.746Z2025-04-15T12:48:25.099ZE-MTAB-15032ERP171650EFO_0002944EFO_0004170EFO_0002693EFO_0004917EFO_0005518EFO_0003816EFO_0004184