biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsChristophe LambingNextSeq 500genotyping by high throughput sequencingArabidopsis thalianaArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15040Genomic DNA from 144 h2a.w7 Col-0 x Ler F2 individuals was extracted using CTAB (as described in Kuo et al, 2022 Methods Mol Biol - PMID: 35461443) and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology - PMID: 31378616). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bethesda) - PMID: 25585881; Yelina et al, 2015 Genes & Dev - PMID: 26494791; Lawrence et al, 2019 Current Biology - PMID: 31378616).biostudies-arrayexpressGrowth Protocol - Plant were grown in growth chambers under the following conditions: 60% humidity, long day conditions (16 hours light/ 8 hours dark) at 20oC.Nucleic Acid Extraction - DNA was extracted using CTAB procedure as previously described (Kuo et al, 2022 Methods Mol Biol - PMID: 35461443)Sample Collection - Leaf tissue at rosette stage were collected from 5-week old plants. Plant tissue was immediately snap frozen in liquid nitrogen and stored at -80oC.Library Construction - DNA library preparation was prepared as previously described (Lawrence et al, 2019 Current Biology - PMID: 31378616) – TruSeq DNA sample prep kit. Illumina sequencingSequencing - Libraries were subjected to paired-end sequenced using an Illumina Genome Analyzer. Illumina NextSeq500 instrument.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesChristophe LambingfalseIdentifying crossover locations in Arabidopsis thaliana h2a.w7 Col-0 × Ler F2 populations using genotyping-by-sequencingGenomic DNA from 144 h2a.w7 Col-0 x Ler F2 individuals was extracted using CTAB (as described in Kuo et al, 2022 Methods Mol Biol - PMID: 35461443) and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology - PMID: 31378616). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bethesda) - PMID: 25585881; Yelina et al, 2015 Genes & Dev - PMID: 26494791; Lawrence et al, 2019 Current Biology - PMID: 31378616).2025-07-11T00:00:00Z2025-04-10T10:27:11.885Z2025-04-10T10:27:11.885ZE-MTAB-15040ERP171467E-MTAB-8725EFO_0002944EFO_0004170EFO_0003789EFO_0002771EFO_0005518EFO_0004184