biostudies-arrayexpressOlivier Van AkenArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15045RNA-seq comparison of the response to touch (by gentle brushing) in two signalling mutants compared to WT. Samples were taken before, and 22 minutes after brushing.biostudies-arrayexpressSample Collection - In vitro grown Plants were either untreated, or brushed gently for about 30 seconds per plate and then collected 22 minutes later. Whole seedlings were collected with tweezers and snap frozen in liquid nitrogen. Samples were then ground to a fine powder while frozen before RNA extractionSample Treatment - Plants were gently brushed with a soft art brush for about 30 seconds per plateLibrary Construction - The library was prepared according to Illumina instructions for mRNA RNA-seqNucleic Acid Extraction - Samples were ground to a fine powder while frozen before RNA extraction. RNA was prepared using the Sigma Spectrum Plant RNA kitGrowth Protocol - Plants were grown for 12 days on 1/2 Murashige Skoog media in a growth chamber with 16h/8h day/night cycle, 100 umol photons -s -m2 at 22 degrees Celcius.Sequencing - Sequencing was done on an Illumina Novaseq with 2x150 bp paired end cycles by the Swedish National Genomics InfrastructureOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSequence Alignment - Reads were aligned to the ARAPORT11 Arabidopsis thaliana genome using STARData Transformation - Data were normalised using DESeq2MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAArabidopsis thalianaOlivier Van AkenfalseRNA-seq of camta123 and mkk45 mutants before and after touchingRNA-seq comparison of the response to touch (by gentle brushing) in two signalling mutants compared to WT. Samples were taken before, and 22 minutes after brushing.2026-06-19T00:00:00Z2026-06-19T01:00:40.109Z2025-04-17T09:35:51.779ZE-MTAB-15045ERP171754EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969