biostudies-arrayexpressPhaewa ChaiwijitHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15054We characterized the different cell types and their differential gene expression at days 10 and 13 of maturation in 2D cortical brain models.biostudies-arrayexpressSample Collection - The iPSC-derived brain cells were trypsinized and dissociated using 0.5% trypsin in PBS, and then resuspended in 0.04% BSA in PBS. A 40-μm strainer was used to remove cell debris and clumps, to obtain single-cell suspension.Library Construction - Single-cell transcriptomic libraries were generated following the Chromium Single-cell 5' Reagent Kits V2 User Guide.Nucleic Acid Extraction - Nucleic acids were extracted following the 10x Genomics Chromium Single Cell 5' Reagent Kits v2 User Guide, up to the cDNA synthesis step. After GEM (Gel Bead-in-Emulsion) generation and reverse transcription, emulsions were broken, and barcoded cDNA was purified using silane magnetic beads, according to the manufacturer’s instructions, with a target recovery of 10,000 cells per sample.Sequencing - Sequencing libraries were generated from 10x Genomics 5' cDNA and sequenced on an Illumina HiSeq X Ten platform. The library from 10-day-old human iPSC-derived brain cells was sequenced across one-third of three lanes, while the library from 13-day-old human iPSC-derived brain cells was sequenced across one-third of a single lane.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Read alignment was performed using Cell Ranger (v6.1.2) against the human reference genome GRCh38. The pipeline generated filtered output matrices, including the gene-barcode matrix (matrix.tsv.gz), corresponding cell barcodes (barcodes.tsv.gz), and gene features (features.tsv.gz).UnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq XRNA-seq of coding RNA from single cellsHomo sapiensVarodom CharoensawanNithi AsavapanumasPhaewa ChaiwijitArunee ThitithanyanontfalseIdentifying GFAP-Expressing Cell Susceptibility to SARS-CoV-2 Infection using Human iPSC-derived neural cellsWe characterized the different cell types and their differential gene expression at days 10 and 13 of maturation in 2D cortical brain models.2026-02-17T00:00:00Z2026-02-17T08:32:41.716Z2025-04-17T10:57:46.431ZE-MTAB-15054ERP171770EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184