{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Dussaud sébastien"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15063"],"description":["The adipose progenitors and the macrophages were sorted from the epididymal adipose tissue of lean (8 weeks chow diet), obese (4 weeks  HFD) and Formerly obese mice (4 weeks  HFD and 4 weeks  chow diet), and their transcriptomic profiles were performed using RNAseq."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche).","Nucleic Acid Extraction - RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen).","Sequencing - RNA-Seq libraries were sequenced on an Illumina NovaSeq 6000.","Sample Collection - Mouse epidydimal adipose tissue was harvested and digested in DMEM and collagenase A. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, the adipocyte fraction is discarded and the stroma vascular fraction passed through a 100 µm filter before staining.Cell suspensions were stained with appropriate antibodies for 30 min on ice.Cell sorting was performed on a BD FACSAria II™ cell sorter."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Dussaud sébastien"],"additional_accession":[]},"is_claimable":false,"name":"Weight loss in visceral adipose tissue","description":"The adipose progenitors and the macrophages were sorted from the epididymal adipose tissue of lean (8 weeks chow diet), obese (4 weeks  HFD) and Formerly obese mice (4 weeks  HFD and 4 weeks  chow diet), and their transcriptomic profiles were performed using RNAseq.","dates":{"release":"2026-09-15T00:00:00Z","modification":"2026-09-16T14:19:59.12Z","creation":"2025-04-17T16:39:05.117Z"},"accession":"E-MTAB-15063","cross_references":{"ENA":["ERP171787"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}