biostudies-arrayexpressPetr JansaMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15076In the Prdm9-driven sterility of Mus m. musculus x Mus m. domesticus hybrids a Prdm9-interacting hybrid sterility gene was mapped in the X-linked Hstx2 locus. Here we show that the Mir465 microRNA gene cluster is the predicted Hstx2 hybrid sterility factor involved in Prdm9 interaction.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated by the Qiagen miRNeasy mini kit (217004), according to the manufacturer's protocol.Library Construction - For the library construction, the KAPA mRNA HyperPrep Kit with Poly(A) mRNA selection (Roche) was used with starting amount of 1000 ng of total RNA.Sequencing - Libraries were sequenced on the Illumina NextSeq® 500 platform (Illumina) using a 75bp single-end configuration. The sequencing yielded on average 30 million reads per sample.Sample Collection - Samples were obtained from testes of juvenile males, and extracted with Qiazol lysis solutionOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSequence Alignment - Technical quality control and gene quantification were done using the nf-core/rnaseq v3.8.1 bioinformatics pipeline (Ewels et al, 2020) with Star mapping (Dobin et al, 2013) and Salmon quantification (Patro et al, 2017) GRCm39 (Ensembl assembly version 104) was selected as the reference genome (Yates et al, 2020).UnknownTranscriptomicsGenomicsProteomicsNextSeq 500RNA-seq of coding RNAMus musculusPetr JansaJiri ForejtLucie PfeiferovafalseTranscriptomics analysis of involvement of miRNA 465 in hybrid mouse sterilityIn the Prdm9-driven sterility of Mus m. musculus x Mus m. domesticus hybrids a Prdm9-interacting hybrid sterility gene was mapped in the X-linked Hstx2 locus. Here we show that the Mir465 microRNA gene cluster is the predicted Hstx2 hybrid sterility factor involved in Prdm9 interaction.2025-12-16T00:00:00Z2025-12-16T10:05:04.757Z2025-04-24T01:15:07.971ZE-MTAB-15076ERP171904EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003738EFO_0004184