{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Anayansi Molina-Hernández"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"organism":["Rattus norvegicus"],"species":["Rattus norvegicus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15111"],"description":["Insight on the understanding of the effect of maternal diabetes on embryonic nervous system development in embryos without neural tube defects. Control Group:  Each sample (n) contained the RNA obtained by TRIZOL of a bulk of 30 prosencephalons (embryo day 12) of embryos from both sexes obtained from pregnant rats that were intraperitoneally injected with citrate buffer. n=3  Experimental group:  Each sample (n) contained the RNA obtained by  TRIZOL of A bulk of 30 prosencephalons (embryo day 12) of embryos from both sexes obtained from pregnant rats that were diabetic induced with an intraperitoneal administration of 50 mg/kg of streptozotocin. n=3  The sequence liberties were obtained through paired-end sequencing experiments.  Codes for the files are as follows:  Control E12:  AM1_S1_R1_001.fastq.gz AM1_S1_R2_001.fastq.gz AM2_S2_R1_001.fastq.gz AM2_S2_R2_001.fastq.gz AM3_S3_R1_001.fastq.gz AM3_S3_R2_001.fastq.gz Diabetic E12:  AM4_S4_R1_001.fastq.gz AM4_S4_R2_001.fastq.gz AM5_S5_R1_001.fastq.gz AM5_S5_R2_001.fastq.gz AM6_S6_R1_001.fastq.gz AM6_S6_R2_001.fastq.gz  Were S = sample, R1 = sense sequencing, and R2 = antisense sequencing.  Sequencing was performed on a NextSeq500 using the TruSeq HT kit from Illumina."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Sequencing was conducted on the Illumina Nextseq 500 platform with paired-end two lectures ×75 bp reads, cycles paired-end to generate approximately 30 million reads per sample. Raw data only; downstream analysis not included in this submission.","Library Construction - RNA-seq libraries were prepared using the Truseq Stranded mRNA library prep kit from (Illumina, CA, USA) according to the manufacturer’s instructions.","Sample Collection - At embryo day 12, pregnant rats were euthanized by decapitation following anesthesia induction with sevoflurane; embryos were collected by cesarian and washed in cold phosphate-buffered saline (PBS, pH 7.4). The hole prosencephalon of embryos without neural tube defects was directed and placed in Trizol for RNA extraction. Embryos of both sexes were used without distinction in all experiments.","Nucleic Acid Extraction - Total RNA was obtained using TRIZOL® reagent (Thermo Fisher Scientific, MA, USA) following provider’s instructions. RNA quality and purity were determined (Nanodrop, Thermo Fisher Scientific), and only 260/230 ≥ 1.0 and 260/280 ≥ 1.8 samples were used. RNA Integrity Number (RIN) was obtained by using a BioAnalyzer (Agilent 2100 BioAnalyzer, CA, USA). The mean RIN was 9.04 ± 0.44"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Anayansi Molina-Hernández"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq from the prosencephalon of 12-day-old embryo from control and diabetic rats","description":"Insight on the understanding of the effect of maternal diabetes on embryonic nervous system development in embryos without neural tube defects. Control Group:  Each sample (n) contained the RNA obtained by TRIZOL of a bulk of 30 prosencephalons (embryo day 12) of embryos from both sexes obtained from pregnant rats that were intraperitoneally injected with citrate buffer. n=3  Experimental group:  Each sample (n) contained the RNA obtained by  TRIZOL of A bulk of 30 prosencephalons (embryo day 12) of embryos from both sexes obtained from pregnant rats that were diabetic induced with an intraperitoneal administration of 50 mg/kg of streptozotocin. n=3  The sequence liberties were obtained through paired-end sequencing experiments.  Codes for the files are as follows:  Control E12:  AM1_S1_R1_001.fastq.gz AM1_S1_R2_001.fastq.gz AM2_S2_R1_001.fastq.gz AM2_S2_R2_001.fastq.gz AM3_S3_R1_001.fastq.gz AM3_S3_R2_001.fastq.gz Diabetic E12:  AM4_S4_R1_001.fastq.gz AM4_S4_R2_001.fastq.gz AM5_S5_R1_001.fastq.gz AM5_S5_R2_001.fastq.gz AM6_S6_R1_001.fastq.gz AM6_S6_R2_001.fastq.gz  Were S = sample, R1 = sense sequencing, and R2 = antisense sequencing.  Sequencing was performed on a NextSeq500 using the TruSeq HT kit from Illumina.","dates":{"release":"2025-05-15T00:00:00Z","modification":"2025-05-02T11:59:48.791Z","creation":"2025-05-02T11:59:48.791Z"},"accession":"E-MTAB-15111","cross_references":{"ENA":["ERP172209"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}