biostudies-arrayexpressMicrotus ochrogasterhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15128This study aimed to uncover changes in gene expression in key brain regions associated with social bonding neuroplasticity in adult prairie voles. The experimental workflow involved the following steps: 1. Male (Ma) and female (Fe) prairie voles were subjected to social cohabitation and mating (SCM) conditions for 120 h, with isolated animals serving as controls. 2. RNA was extracted and analyzing RNA from three brain regions crucial for social bonding and neuroplasticity: the subventricular zone (SVZ), dentate gyrus (DG), and nucleus accumbens (NAc). 3. RNA sequencing was performed to identify differentially expressed genes between the SCM and control groups. 4. Gene ontology (GO) and functional enrichment analyses were performed to understand the biological processes involved in pair bonding.   5. Validation of key findings through quantitative PCR and in vitro neurosphere assays using cells isolated from the SVZ. This approach allowed the examination of sex-specific changes in gene expression related to neurogenesis, synaptic plasticity, and other probable molecular pathways involved in pair bond formation, with potential implications for understanding human relationships and related neuropsychiatric conditions.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated from each sample using TRIzol and DNase I.RNA integrity was assessed using a Agilent BioAnalyzer, retaining only samples with an RNA integrity number (RIN) >8.0.Sample Collection - Subjects were euthanized with an overdose of pentobarbital (6.3 mg/vol) by intraperitoneal (IP) administration, and the whole brains were obtained to dissect the SVZ, DG, and NAc tissues. Briefly, whole brains were collected in Petri dishes with an ice-cold solution of 1X PBS, 2 mM HEPES, 20 mM glucose, and 25 mM NaHCO3. The three brain regions were dissected under a stereoscope and immediately frozen.Library Construction - Libraries were constructed using the Truseq Stranded mRNA library prep kit according to the manufacturer’s instruction (Illumina).Sequencing - Sequencing was performed on an Illumina NextSeq 500 platform at the Instituto de Biotecnologia, UNAM, using paired-end 76-bp reads.MINSEQE ScoreAssays and DataProcessed DataorganisationMAGE-TAB FilesData Transformation - The reads were aligned to the Microtus_ochrogaster genome assembly (GCF_000317375.1) using SMALT (v0.7.6) with default parameters. Gene coverage was determined using coverageBed (BEDtools v2-2.27.1). A custom Perl script was then used to generate a gene count matrix (i.e., table of abundances), including only genes with non-zero counts and >25% length coverage. Each count matrix file was processed using the public Galaxy server (https://usegalaxy.org/) for quality control, sample distribution analysis and differential expression (DE) analysis (Bioconductor limma-voom)27. Genes with a Log Fold Change  1.5 and p- adjusted  0.05 were deemed differentially expressed.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 500RNA-seq of coding RNAMicrotus ochrogasterERP172396Néstor Fabián DíazWendy PortilloDaniela Ávila-GonzálezfalseRNA-seq of brain tissue from the subventricular zone, dentate gyrus and nucleus accumbens of adult male and female voles undergoing pair bond formationThis study aimed to uncover changes in gene expression in key brain regions associated with social bonding neuroplasticity in adult prairie voles. The experimental workflow involved the following steps: 1. Male (Ma) and female (Fe) prairie voles were subjected to social cohabitation and mating (SCM) conditions for 120 h, with isolated animals serving as controls. 2. RNA was extracted and analyzing RNA from three brain regions crucial for social bonding and neuroplasticity: the subventricular zone (SVZ), dentate gyrus (DG), and nucleus accumbens (NAc). 3. RNA sequencing was performed to identify differentially expressed genes between the SCM and control groups. 4. Gene ontology (GO) and functional enrichment analyses were performed to understand the biological processes involved in pair bonding.   5. Validation of key findings through quantitative PCR and in vitro neurosphere assays using cells isolated from the SVZ. This approach allowed the examination of sex-specific changes in gene expression related to neurogenesis, synaptic plasticity, and other probable molecular pathways involved in pair bond formation, with potential implications for understanding human relationships and related neuropsychiatric conditions.2025-05-15T00:00:00Z2026-05-27T17:54:21.007Z2025-05-07T21:46:01.98ZE-MTAB-15128ERP172396EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184