{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15132"],"description":["This study investigates the preclinical effects of Tumor Treating Fields (TTFields) in pancreatic ductal adenocarcinoma (PDAC)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - For cDNA libraries preparation, the polyA fraction (mRNA) was purified, followed by fragmentation and generation of double-stranded cDNA. Agencourt AMPure XP beads (Beckman Coulter) were used for cleanup, followed by end repair, UMIs addition, adapter ligation and PCR amplification steps.","Sample Collection - Samples were fresh frozen in TRIzol","Sample Treatment - TTFields were applied to cells using the inovitroTM system as previously described (Kirson, E.D., et al., Disruption of cancer cell replication by alternating electric fields. Cancer Res, 2004. 64(9): p. 3288-95.) With frequency of 150 kHz and intensity of 1.7 V/cm RMS for 24 and 48 hours.","Nucleic Acid Extraction - RNA was purified with TRI reagent as previously described [ Rio, D.C., et al., Purification of RNA Using TRIzol (TRI Reagent). Cold Spring Harbor Protocols, 2010. 2010(6): p. pdb.prot5439. ]","Growth Protocol - Grown in a 37 °C humidified incubator in RPMI1640 media supplemented with 10% FBS. All media were further supplemented with 2 mM L-glutamine and 50 μg/ml penicillin/streptomycin.","Sequencing - Sequencing was done on Illumina NovoSeq 6000 system."],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"additional_accession":["ERP172427"],"pubmed_authors":["Kerem Wainer Katsir"]},"is_claimable":false,"name":"RNA-seq of pancreatic cell lines AsPC1 and BxPC3 treated with Tumor Treating Fields (TTFields) compared to control samples","description":"This study investigates the preclinical effects of Tumor Treating Fields (TTFields) in pancreatic ductal adenocarcinoma (PDAC).","dates":{"release":"2025-12-30T00:00:00Z","modification":"2025-12-30T02:02:11.666Z","creation":"2025-05-08T14:35:39.958Z"},"accession":"E-MTAB-15132","cross_references":{"ENA":["ERP172427"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003738","EFO_0004184","EFO_0003969"]}}