biostudies-arrayexpressHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15132This study investigates the preclinical effects of Tumor Treating Fields (TTFields) in pancreatic ductal adenocarcinoma (PDAC).biostudies-arrayexpressLibrary Construction - For cDNA libraries preparation, the polyA fraction (mRNA) was purified, followed by fragmentation and generation of double-stranded cDNA. Agencourt AMPure XP beads (Beckman Coulter) were used for cleanup, followed by end repair, UMIs addition, adapter ligation and PCR amplification steps.Sample Collection - Samples were fresh frozen in TRIzolSample Treatment - TTFields were applied to cells using the inovitroTM system as previously described (Kirson, E.D., et al., Disruption of cancer cell replication by alternating electric fields. Cancer Res, 2004. 64(9): p. 3288-95.) With frequency of 150 kHz and intensity of 1.7 V/cm RMS for 24 and 48 hours.Nucleic Acid Extraction - RNA was purified with TRI reagent as previously described [ Rio, D.C., et al., Purification of RNA Using TRIzol (TRI Reagent). Cold Spring Harbor Protocols, 2010. 2010(6): p. pdb.prot5439. ]Growth Protocol - Grown in a 37 °C humidified incubator in RPMI1640 media supplemented with 10% FBS. All media were further supplemented with 2 mM L-glutamine and 50 μg/ml penicillin/streptomycin.Sequencing - Sequencing was done on Illumina NovoSeq 6000 system.MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensERP172427Kerem Wainer KatsirfalseRNA-seq of pancreatic cell lines AsPC1 and BxPC3 treated with Tumor Treating Fields (TTFields) compared to control samplesThis study investigates the preclinical effects of Tumor Treating Fields (TTFields) in pancreatic ductal adenocarcinoma (PDAC).2025-12-30T00:00:00Z2025-12-30T02:02:11.666Z2025-05-08T14:35:39.958ZE-MTAB-15132ERP172427EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184EFO_0003969