{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Laurie Nemoz-Billet"],"organism":["Danio rerio"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15160"],"description":["In this study, we performed single-cell RNA-sequencing (scRNA-seq) on 24 hours post-fertilization (hpf) mnx1:GFP zebrafish embryos to characterize the transcriptional diversity of developing spinal motor neurons. Our goal was to identify molecularly distinct subtypes within the motor neuron population. This dataset provides evidence for the existence of at least three transcriptionally distinct motor neuron subtypes."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Trunks dissociated and GFP cells selected by FACS","Nucleic Acid Extraction - 10X nucleic acid library construction protocol","Library Construction - Chromium next GEM single cell 3' reagent kit v3.1","Sequencing - NextSeq500 Illumina Sequencer High sequencing run"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Normalization using Seurat v4.3.0","Sequence Alignment - Reads analysed with the Cell Ranger v6.0.1"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Danio rerio"],"pubmed_title":["Single-cell transcriptomic profiling of zebrafish primary motor neurons reveals subtype-specific gene expression signatures"],"pubmed_authors":["Laurie Nemoz-Billet, Nathan Gil, Chloé Exbrayat-Héritier, Mathilde Bouchet, Marilyne Malbouyres, Sandrine Hughes, Benjamin Gillet, Jonathan Enriquez, Sandrine Bretaud, Florence Ruggiero","Laurie Nemoz-Billet"],"additional_accession":[]},"is_claimable":false,"name":"scRNA-seq of GFP+ cells isolated from developing 24 hpf mnx1:GFP zebrafish embryos","description":"In this study, we performed single-cell RNA-sequencing (scRNA-seq) on 24 hours post-fertilization (hpf) mnx1:GFP zebrafish embryos to characterize the transcriptional diversity of developing spinal motor neurons. Our goal was to identify molecularly distinct subtypes within the motor neuron population. This dataset provides evidence for the existence of at least three transcriptionally distinct motor neuron subtypes.","dates":{"release":"2025-11-11T00:00:00Z","modification":"2025-11-11T18:16:40.799Z","creation":"2025-05-21T14:12:24.444Z"},"accession":"E-MTAB-15160","cross_references":{"ENA":["ERP172807"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0004184"]}}