biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsCatherine OryIllumina HiSeq 2000RNA-seq of total RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15168We compared the molecular specificities of tumours developed in Ukrainian subjects exposed or not to radioiodines during the Chernobyl accident. Exposed subjects are associated with estimated high thyroid doses (0.58 to 4.5 Gy) known to be associated with an increased risk of developing radiation-induced thyroid cancer, or with low doses (21 to 40 mGy) below the significance limit of conventional epidemiology. Corresponding non-pathological tissues, whether exposed or not, adjacent to the tumours were also analysed. Most of the samples were also analyzed for the MiRNome (E-MTAB-10094) or the exome (E-MTAB-10931).biostudies-arrayexpressSequencing - Sequencing has been performed on a Sequencing has been performed on a Illumina HiSeq2000 as paired-end 100 bp reads, using Illumina sequencing reagents. Libraries were generally pooled by 4 samples per lane to generate on average around 40 to 50 million reads in total per sample. Fastq files produced after RNA-seq sequencing have been processed by in-house CNRGH tools to assess quality of raw and genomic-aligned nucleotides. as paired-end 100 bp reads, using Illumina sequencing reagents. Libraries were generally pooled by 4 samples per lane to generate on average around 40 to 50 million reads in total per sample. Fastq files produced after RNA-seq sequencing have been processed by in-house CNRGH tools to assess quality of raw and genomic-aligned nucleotides.Sample Collection - The Chernobyl Tissue Bank (CTB) provided the post-Chernobyl series of thyroid tumors and non tumoral tissues adjacent to the tumors, as total RNA samples. All informations used for samples annotation were provided by the CTB. The confirmation of diagnosis was performed by the International Pathology Panel of the CTB, which is supported by NCI grant number U24CA082102: Dr A Abrosimov, Pr T Bogdanova, Pr G Fadda, Professor J Hunt, Pr M Ito, Pr V LiVolsi, Pr J Rosai, Pr ED Williams. Tumors and adjacent non tumoral tissues were considered as radiation-induced and exposed, respectively, when the patients were under 15 years old (period of high thyroid radiation sensitivity), and lived in oblasts of Ukraine highly contaminated at the time of the Chernobyl accident. The series also included Ukrainian patients, born in the same oblasts than radiation-induced cases after March 1987 and therefore considered at as non-exposed (sporadic PTC) due to radioactive iodines short decay.Nucleic Acid Extraction - Total RNA samples were povided by the CTB purified using QIAGEN columnsLibrary Construction - Librairy contruction and total Stranded RNAseq sequencing were performed by the Centre National de Recherche en Génomique Humaine (CNRGH), Institut de Biologie François Jacob, CEA, Evry, FRANCE. After complete RNA quality control on each sample (quantification in duplicate on a NanoDrop™ 8000 spectrophotometer and RNA6000 Nano LabChip analysis on Bioanalyzer from Agilent), libraries have been prepared using the “Total RNA Library Prep Kit with Ribo-Zero” from Illumina, which removes cytoplasmic rRNA as a first step of library preparation. An input of 500 ng total RNA was used for all samples, and libraries were prepared on an automated platform, according to manufacturer’s instructions. Library quality has been checked by LabGx (Perkin Elmer) analysis for profile analysis and quantification, and sample libraries have then been pooled before sequencing to reach the expected sequencing depth.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesCatherine OryfalseRNA Seq analysis of thyroid tumors and adjacent non tumoral tissues of Ukrainian patients exposed or not to Chernobyl radioiodinesWe compared the molecular specificities of tumours developed in Ukrainian subjects exposed or not to radioiodines during the Chernobyl accident. Exposed subjects are associated with estimated high thyroid doses (0.58 to 4.5 Gy) known to be associated with an increased risk of developing radiation-induced thyroid cancer, or with low doses (21 to 40 mGy) below the significance limit of conventional epidemiology. Corresponding non-pathological tissues, whether exposed or not, adjacent to the tumours were also analysed. Most of the samples were also analyzed for the MiRNome (E-MTAB-10094) or the exome (E-MTAB-10931).2026-02-24T00:00:00Z2026-02-24T09:25:31.38Z2025-05-27T11:37:50.265ZE-MTAB-15168ERP172941E-MTAB-10931E-MTAB-10094EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184