biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsFinetti Pascaltranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15171Since the discovery of the JAK2V617F tyrosine kinase-activating mutation several genes have been found mutated in nonchronic myeloid leukemia (CML) myeloproliferative neoplasms (MPNs), which mainly comprise three subtypes of \"classic\" MPNs; polycythemia vera (PV), essential thrombocythemia (ET), and myelofibrosis (MF). We searched for specific molecular profil associated to PV, ET and MF.biostudies-arrayexpressSample Collection - Patient's samples were obtained after informed consent in accordance with the Declaration of Helsinki and stored at Institut Paoli-Calmettes/Centre de Recherche en Cancérologie de Marseille (IPC/CRCM) Tumor Bank repository.Labeling - Preparation of cRNA was done as recommended by the supplier with the “GeneChip® WT PLUS Reagent Kit (Affymetrix)“ for HUGENE ST 2.0 and \"GeneChip 3’ IVT Express Kit (Affymetrix)\" for HGU133+2.0 gene chips.Scaning - Detection and quantification were performed as recommended by the supplier using GeneChip Scanner 3000 7G (Affymetrix).Nucleic Acid Extraction - RNA was treated with RNase-free DNase set (Qiagen) to remove contaminating genomic DNAHybridization - Hybridizations and washes were performed as recommended by the supplier.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsFinetti PascalData Transformation - Data from both gene chip types were normalized separately by the Robust Multichip Average method. Next, we mapped hybridization probes across the technological microarray platforms represented in both data sets, where all probes were mapped based on their NCBI/'Entrez Gene ID' and, in the case of multiple probes, mapped to the same 'Entrez Gene ID', the one with the highest variance. After batch normaisation using 'inSilicoMerging' R/package, both normalized data sets where gathered together.falseGene expression-based rationale in primitive myelofibrosisSince the discovery of the JAK2V617F tyrosine kinase-activating mutation several genes have been found mutated in nonchronic myeloid leukemia (CML) myeloproliferative neoplasms (MPNs), which mainly comprise three subtypes of \"classic\" MPNs; polycythemia vera (PV), essential thrombocythemia (ET), and myelofibrosis (MF). We searched for specific molecular profil associated to PV, ET and MF.2025-06-20T00:00:00Z2025-05-28T10:56:33.279Z2025-05-28T10:56:33.279ZE-MTAB-15171EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815