{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Andreas Agrafiotis"],"instrument_platform":["Diva","Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15175"],"description":["We performed single-cell transcriptome and antibody repertoire sequencing of bone marrow B cells from mice following influenza (H1N1 and H3N2) intranasal infections"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - 10x genomics 5' protocol was performed as according to manufacturers","Sequencing - Paired-end sequencing of libraries with Illumina NovaSeq S1","Sample Collection - Female 6-8 weeks old C57BL/6 mice (Janvier) were infected with 200 PFU of PR8 (A/PuertoRico/8/1934 (H1N1)) or X31 (a reassortant virus that contains the six internal segments from PR8 and the glycoprotein-encoding segments (HA/NA) from strain A/Aichi/1968 (H3N2)) (LD50 = 1250 PFU) intranasally (i.n.). the tibia and femur bones were flushed in ice-cold RPMI containing 10% FCS buffer with 10 ng/mL IL6 (216-16, Peprotech). An RBC lysis step was performed in 4 mL ammonium-chloride-potassium lysis buffer for 1 min at room temperature and subsequently inactivated with 26 mL RPMI containing 10% FCS. Cells were isolated via facs following the aforementioned experimental conditions","Nucleic Acid Extraction - 10x genomics 5' protocol was performed as according to manufacturers"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Andreas Agrafiotis"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell transcriptome and antibody repertoire sequencing of bone marrow B cells from mice following influenza (H1N1 and H3N2) intranasal infections","description":"We performed single-cell transcriptome and antibody repertoire sequencing of bone marrow B cells from mice following influenza (H1N1 and H3N2) intranasal infections","dates":{"release":"2025-06-19T00:00:00Z","modification":"2025-05-30T12:30:42.129Z","creation":"2025-05-30T12:30:42.129Z"},"accession":"E-MTAB-15175","cross_references":{"ENA":["ERP173056"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}