biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsAndreas AgrafiotisDivaIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15175We performed single-cell transcriptome and antibody repertoire sequencing of bone marrow B cells from mice following influenza (H1N1 and H3N2) intranasal infectionsbiostudies-arrayexpressLibrary Construction - 10x genomics 5' protocol was performed as according to manufacturersSequencing - Paired-end sequencing of libraries with Illumina NovaSeq S1Sample Collection - Female 6-8 weeks old C57BL/6 mice (Janvier) were infected with 200 PFU of PR8 (A/PuertoRico/8/1934 (H1N1)) or X31 (a reassortant virus that contains the six internal segments from PR8 and the glycoprotein-encoding segments (HA/NA) from strain A/Aichi/1968 (H3N2)) (LD50 = 1250 PFU) intranasally (i.n.). the tibia and femur bones were flushed in ice-cold RPMI containing 10% FCS buffer with 10 ng/mL IL6 (216-16, Peprotech). An RBC lysis step was performed in 4 mL ammonium-chloride-potassium lysis buffer for 1 min at room temperature and subsequently inactivated with 26 mL RPMI containing 10% FCS. Cells were isolated via facs following the aforementioned experimental conditionsNucleic Acid Extraction - 10x genomics 5' protocol was performed as according to manufacturersOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesAndreas AgrafiotisfalseSingle-cell transcriptome and antibody repertoire sequencing of bone marrow B cells from mice following influenza (H1N1 and H3N2) intranasal infectionsWe performed single-cell transcriptome and antibody repertoire sequencing of bone marrow B cells from mice following influenza (H1N1 and H3N2) intranasal infections2025-06-19T00:00:00Z2025-05-30T12:30:42.129Z2025-05-30T12:30:42.129ZE-MTAB-15175ERP173056EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184