biostudies-arrayexpressRajiv SanwalHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15177Uninfected and influenza (H1N1, x31) conditions were investigated. Three biological replicates were used per group for RNASeq analysis. A non functional derivative of Aeroplysinin-1 was also tested (Ap1) at the same concentration and time point. Samples had an Agilent Bioanalyzer RNA integrity number above 7 and an OD260/230 ratio of 2.0-2.2. Library prep was performed using the NEB Ultra II Directional mRNA kit. Sequencing was performed with the Illumina Hiseq2500 platform with high throughput flowcell PE2x125bp (paired end 2x125 base pair configuration), with 25-30 million reads per sample.biostudies-arrayexpressSample Collection - Total RNA was isolated from human primary microvascular endothelial cells (HPMECs) seeded on 6 well plates (3.8 x 105cells/well). HPMECs were infected with influenza and treated with DMSO, Ap (1 μM) or Ap-1 (a modified inactive derivative of Ap) for two hours.Library Construction - Library prep was performed using the NEB Ultra II Directional mRNA kit.Sequencing - Sequencing was performed with the Illumina Hiseq2500 platform with high throughput Flowcell PE2x125bp (paired end 2x125 base pair configuration), with 25-30 million reads per sample.Nucleic Acid Extraction - Lysates were prepared by washing cells twice with PBS+, lysed in recommended volume of RLT Lysis buffer from the Qiagen RNeasy RNA Isolation Kit (Cat. No. / ID: 74104) and collected by scraping with a sterile cell scraper. Lysates were vortexed for 20 seconds, centrifuged at max speed for 3 minutes at room temperature at stored at -80C for up to two weeks prior to extraction with the kit which was performed according to manufacturer’s instructions.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - The edgeR package v3.22.3 (http://www.bioconductor.org/packages/release/bioc/html/edgeR.html) was used for FPKM generation, filtering and normalization of the complete data set. At the filtering step genes with FPKM > 2 in at least 2 samples were retained. Thisisintendedtoremovegenesthatarenotexpressed, or expressed at a very low level. The method used for normalizing the data was TMM, implemented by the calcNormFactors(y) function.UnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 2500RNA-seq of coding RNAHomo sapiensAlexandre HardyRajiv SanwalElyse LatreilleBhooma ThiruvahindrapuramWarren LeefalseRNA-Seq: Aeroplysinin-1 on gene expression in primary human lung endothelial cells with and without influenza AUninfected and influenza (H1N1, x31) conditions were investigated. Three biological replicates were used per group for RNASeq analysis. A non functional derivative of Aeroplysinin-1 was also tested (Ap1) at the same concentration and time point. Samples had an Agilent Bioanalyzer RNA integrity number above 7 and an OD260/230 ratio of 2.0-2.2. Library prep was performed using the NEB Ultra II Directional mRNA kit. Sequencing was performed with the Illumina Hiseq2500 platform with high throughput flowcell PE2x125bp (paired end 2x125 base pair configuration), with 25-30 million reads per sample.2025-06-14T00:00:00Z2025-05-29T09:23:12.801Z2025-05-29T09:23:12.801ZE-MTAB-15177ERP173002EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184