{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Martin Simon"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of coding RNA"],"organism":["Caenorhabditis elegans"],"species":["Caenorhabditis elegans"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15180"],"description":["In this study, we demonstrate the consequences of Fe overdosing (achieved by iron(III) ammonium citrate (FAC)) or/and GSH depletion (achieved by the GSH binding molecule diethyl maleate (DEM)) in Caenorhabditis elegans (C. elegans) on Fe homeostasis."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - The worms were cultivated on 8P agar plates coated with NA22 E. coli at 20 °C, 750 worms were isolated after treatment for RNA isolation.","Library Construction - Library preparation was performed with 300 ng RNA using the NEBNext® Ultra™ II directional RNA library prep kit for Ilumina® with 14 PCR cycles according to manufacturers instructions.","Nucleic Acid Extraction - RNA was isolated from 750 worms in 1 mL TRI reagent® (Sigma-Aldrich). Isolated RNA was purified with the RNA clean and concentrator™-5 Kit (Zymo Research) according to the manufacturers instructions.","Sequencing - Librararies were pooled and sequenced on a Illumina NovaSeqX Platform using 1.5B FC."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Martin Simon"],"additional_accession":[]},"is_claimable":false,"name":"C.elegans iron homoeostasis","description":"In this study, we demonstrate the consequences of Fe overdosing (achieved by iron(III) ammonium citrate (FAC)) or/and GSH depletion (achieved by the GSH binding molecule diethyl maleate (DEM)) in Caenorhabditis elegans (C. elegans) on Fe homeostasis.","dates":{"release":"2025-06-14T00:00:00Z","modification":"2025-05-29T11:38:44.786Z","creation":"2025-05-29T11:38:44.786Z"},"accession":"E-MTAB-15180","cross_references":{"ENA":["ERP173007"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}