biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMartin SimonIllumina NovaSeq XRNA-seq of coding RNACaenorhabditis elegansCaenorhabditis eleganshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15180In this study, we demonstrate the consequences of Fe overdosing (achieved by iron(III) ammonium citrate (FAC)) or/and GSH depletion (achieved by the GSH binding molecule diethyl maleate (DEM)) in Caenorhabditis elegans (C. elegans) on Fe homeostasis.biostudies-arrayexpressSample Collection - The worms were cultivated on 8P agar plates coated with NA22 E. coli at 20 °C, 750 worms were isolated after treatment for RNA isolation.Library Construction - Library preparation was performed with 300 ng RNA using the NEBNext® Ultra™ II directional RNA library prep kit for Ilumina® with 14 PCR cycles according to manufacturers instructions.Nucleic Acid Extraction - RNA was isolated from 750 worms in 1 mL TRI reagent® (Sigma-Aldrich). Isolated RNA was purified with the RNA clean and concentrator™-5 Kit (Zymo Research) according to the manufacturers instructions.Sequencing - Librararies were pooled and sequenced on a Illumina NovaSeqX Platform using 1.5B FC.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMartin SimonfalseC.elegans iron homoeostasisIn this study, we demonstrate the consequences of Fe overdosing (achieved by iron(III) ammonium citrate (FAC)) or/and GSH depletion (achieved by the GSH binding molecule diethyl maleate (DEM)) in Caenorhabditis elegans (C. elegans) on Fe homeostasis.2025-06-14T00:00:00Z2025-05-29T11:38:44.786Z2025-05-29T11:38:44.786ZE-MTAB-15180ERP173007EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184