biostudies-arrayexpressChristophe RACHEZMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15197Chromatin accessibility assay by ATACseq in BMEL cells. Three clones (TKO1, TKO2, TKO3) of BMEL TKO cells invalidated for the expression of the three HP1 isoforms (Cbx1-/-; Cbx3-/-; Cbx5-/-) were compared to three clones (Het1, Het32, Het35) of BMEL Het cells (Cbx1+/+; Cbx3+/+; Cbx5+/-) as controls.biostudies-arrayexpressLibrary Construction - Chromatin accessibility assays, including library construction, on Het1, Het32, Het35, TKO1, TKO2, TKO3 were performed with an ATACseq kit (ActiveMotif, 53150) according to the manufacturer's instructions.Sequencing - Paired-end sequencing (9G raw data/sample) of the dual-index ATACseq libraries was performed by Novogene (UK) Co., Ltd.Sample Collection - Chromatin accessibility assays on Het1, Het32, Het35, TKO1, TKO2, TKO3 were performed with an ATACseq kit (ActiveMotif, 53150) according to the manufacturer's instructions.Nucleic Acid Extraction - Chromatin accessibility assays, including nucleic acid extraction, on Het1, Het32, Het35, TKO1, TKO2, TKO3 were performed with an ATACseq kit (ActiveMotif, 53150) according to the manufacturer's instructions.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Bigwig files were generated from BAM files with bamCoverage (parameter: –normalizeUsing CPM) from Deeptools (v3.1.3)Sequence Alignment - ATACseq reads were mapped to mm10 using bowtie2 with the parameters --very-sensitive -X 2000 --maxins 500 --no-discordant --no-mixed -k 1. Aligned BAM files were sorted and indexed using SAMtools (v1.8).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XATAC-seqMus musculusChristophe RACHEZfalseATACseq in BMEL cells invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)Chromatin accessibility assay by ATACseq in BMEL cells. Three clones (TKO1, TKO2, TKO3) of BMEL TKO cells invalidated for the expression of the three HP1 isoforms (Cbx1-/-; Cbx3-/-; Cbx5-/-) were compared to three clones (Het1, Het32, Het35) of BMEL Het cells (Cbx1+/+; Cbx3+/+; Cbx5+/-) as controls.2026-03-31T00:00:00Z2026-03-31T01:03:46.847Z2025-06-04T19:05:07.363ZE-MTAB-15197ERP173170E-MTAB-15196E-MTAB-15194EFO_0002944EFO_0007045EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0004184