biostudies-arrayexpressDomenico PalumboHomo sapiensSTARDESeq2https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15198In this study, we performed the transcriptome analysis of a cohort comprising 72 (20 already published E-MTAB-11599) MTC (medullary thyroid carcinoma) specimens from patients with different genetic background (w/o RET or RAS mutations).biostudies-arrayexpressNucleic Acid Extraction - Total RNA was extracted from fresh-frozen tissue samples using the Norgen Kit (Biotek Corp, Ontario, Canada), following the manufacturer’s instructions.Library Construction - Libraries preparation was performed by using the TruSeq Stranded Total RNA kit (Cat. 20020598, Illumina) according to manufacturer’s guidelines and starting from 200 ng of input material.Sample Collection - Patients' tissues collection was performed at Padua Hospital during surgery. The samples were frozen at -80 C° before RNA extraction.Sequencing - Libraries were sequenced on NextSeq 500 platform (Illumina) in paired end mode (2 × 75 bp).OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - DESeq2 package (v1.44.0) in R was employed for detection of differentially expressed genes. Genes with a sum of counts less than 10 in all the samples were removed and the data were normalized taking into account sex and batch as variables. Genes were considered differentially expressed if they showed |FC| ≥ 1.5 and adjusted p-value ≤ 0.05.Sequence Alignment - Alignment was performed against human genome version hg38 (gencode release 37) with STAR (v2.7.11b) and expressed genes were identified using featureCounts (v2.0.1).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsTruSeq Stranded Total RNA kitNorgen Kit (Biotek Corp)NextSeq 500RNA-seq of coding RNAHomo sapiensComprehensive Transcriptome Profiling of Sporadic Medullary Thyroid CarcinomaDomenico PalumboDomenico Palumbo, Viola Melone, Luigi Palo, Fabio Russo, Domenico Rocco, Caterina Mian, Susi Barollo, Simona Censi, Loris Bertazza, Maria Chiara Zatelli, Alessandro Weisz, Roberta Tarallo, Mario VitalefalseComprehensive Transcriptome Profiling of Sporadic Medullary Thyroid CarcinomaIn this study, we performed the transcriptome analysis of a cohort comprising 72 (20 already published E-MTAB-11599) MTC (medullary thyroid carcinoma) specimens from patients with different genetic background (w/o RET or RAS mutations).2026-01-07T00:00:00Z2026-01-07T10:19:39.03Z2025-06-04T19:47:40.76ZE-MTAB-15198ERP173171E-MTAB-11599EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184www.doi.org/10.1038/s41597-026-06550-7