{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Dr. Santosh Chauhan"],"instrument_platform":["Illumina HiSeq 4000"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15207"],"description":["To understand the transcriptomic changes upon LLOMe treatment at different time points."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - The library was sequenced using the HiSeq 4000 Illumina platform.","Nucleic Acid Extraction - RNA was extracted from MEF cells (three biological replicates) using RNeasy mini kit (QIAGEN, #74104). The quality and quantity of total RNA was checked using agarose gel and Qubit 4.0.","Library Construction - After assessing the quality of RNA, 800-900 ng of total RNA was subjected to NEBNext® ultra™ directional RNA library prep kit for Illumina® (#E7420) using NEBNextPoly(A) mRNA magnetic isolation module (NEB #E7490). The QC of the prepared library was performed using High-Sensitivity Tape Station Kit (Agilent 2200, 5067-5585 (reagents) #5067-5584 ( screen tapes)) for fragment length distribution and Qubit dsDNA HS assay kit (Invitrogen, Q32851) for quantifying the library.","Sample Collection - Untreated and treated MEF cells were collected from 3 different 10cm plates for each group"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Dr. Santosh Chauhan"],"additional_accession":[]},"is_claimable":false,"name":"RNA-Seq of MEF cells treated with LLOMe","description":"To understand the transcriptomic changes upon LLOMe treatment at different time points.","dates":{"release":"2025-07-04T00:00:00Z","modification":"2025-07-04T08:07:10.559Z","creation":"2025-06-05T10:23:21.354Z"},"accession":"E-MTAB-15207","cross_references":{"ENA":["ERP173190"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}