biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsDr. Santosh ChauhanIllumina HiSeq 4000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15207To understand the transcriptomic changes upon LLOMe treatment at different time points.biostudies-arrayexpressSequencing - The library was sequenced using the HiSeq 4000 Illumina platform.Nucleic Acid Extraction - RNA was extracted from MEF cells (three biological replicates) using RNeasy mini kit (QIAGEN, #74104). The quality and quantity of total RNA was checked using agarose gel and Qubit 4.0.Library Construction - After assessing the quality of RNA, 800-900 ng of total RNA was subjected to NEBNext® ultra™ directional RNA library prep kit for Illumina® (#E7420) using NEBNextPoly(A) mRNA magnetic isolation module (NEB #E7490). The QC of the prepared library was performed using High-Sensitivity Tape Station Kit (Agilent 2200, 5067-5585 (reagents) #5067-5584 ( screen tapes)) for fragment length distribution and Qubit dsDNA HS assay kit (Invitrogen, Q32851) for quantifying the library.Sample Collection - Untreated and treated MEF cells were collected from 3 different 10cm plates for each groupOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesDr. Santosh ChauhanfalseRNA-Seq of MEF cells treated with LLOMeTo understand the transcriptomic changes upon LLOMe treatment at different time points.2025-07-04T00:00:00Z2025-07-04T08:07:10.559Z2025-06-05T10:23:21.354ZE-MTAB-15207ERP173190EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184