biostudies-arrayexpressPekka RappuMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15211To study function of Embigin in mouse embryos, E13.5 WT (four individuals per replicate) and Emb-/- (six individuals per replicate) kidneys were dissected, and the RNA was isolated and submitted to high-throughput sequencing to find differentially expressed genes between WT and Emb knock-out mouse lungs or placentasbiostudies-arrayexpressSample Collection - E13.5 WT (four individuals per replicate) and Emb-/- (six individuals per replicate) kidneys were dissectedSequencing - Sequencing was performed with NovaSeq 6000 SP Sequencing System (Illumina) using paired-end sequencing chemistry and 2 x 50 bp read length. The reads obtained from the instrument were base called using bcl2fastq2 conversion software. Raw data were obtained as fastq-files.Library Construction - The libraries were prepared from 300 ng of RNA from each sample using TruSeq Stranded mRNA HT Kit and TruSeq Stranded mRNA Sample Preparation protocol 15031047 (Illumina)Nucleic Acid Extraction - Total RNA was isolated by using the NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s instructions.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - The reads were aligned against the reference genome (Mus musculus GRCm38.95, available in Chipster) using STAR, version 2.7.3 (Dobin et al., 2013)Data Transformation - The reads associated with each gene were counted using the HTSeq package, version 0.12.4.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusPekka RappuJyrki HeinofalseRNA-seq of E13.5 WT and Emb-/- kidneyTo study function of Embigin in mouse embryos, E13.5 WT (four individuals per replicate) and Emb-/- (six individuals per replicate) kidneys were dissected, and the RNA was isolated and submitted to high-throughput sequencing to find differentially expressed genes between WT and Emb knock-out mouse lungs or placentas2026-02-10T00:00:00Z2026-02-10T06:43:12.418Z2025-06-09T11:18:14.836ZE-MTAB-15211ERP173278EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184