{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Zhe Yang Yim"],"instrument_platform":["NextSeq 550"],"study_type":["MBD-seq"],"organism":["Acyrthosiphon pisum"],"species":["Acyrthosiphon pisum"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15212"],"description":["Different pea aphid response differently to stress response with certain genotype producing wing but others changing body colour morph, However, the underlying epigenetic mechanisms remains unclear. Therefore, we used MBD-seq to analyse the epigenomic profile of the four different pea aphid morphs (N116 winged, N116 wingless, N127 red,, N127 pale) to provide insights into the epigenetic profiling (DNA methylation) in regulating this polyphenism. Alternative morphs (N116 winged and N127 pale) were obtained through crowding experiment."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Samples were collected from the crowding experiment that is repeat 7 times.","Nucleic Acid Extraction - Each biological replicate represent seven adult aphid (pooled samples of 1 aphid from each crowding experiment). DNA were extracted using the Qiagen DNAeasy Blood and Tissue Kit","Sequencing - Library is sequenced on the illumina Next Seq 550 series using the NextSeq 500/550 v2.5Kits (75 cycle) and output data was demultiplex (allowing one mistmatch) and BCL to Fastq conversion were performed using illumina bcl2fastq software version 2.20.0.44","Library Construction - Library were constructed using the Next Gen DNA library kit (Active motif) and each samples were added a specific index using the NextGen Indexing Kit"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Zhe Yang Yim"],"additional_accession":[]},"is_claimable":false,"name":"MBD-seq for 4 different pea aphid morphs to understanding polyphenism","description":"Different pea aphid response differently to stress response with certain genotype producing wing but others changing body colour morph, However, the underlying epigenetic mechanisms remains unclear. Therefore, we used MBD-seq to analyse the epigenomic profile of the four different pea aphid morphs (N116 winged, N116 wingless, N127 red,, N127 pale) to provide insights into the epigenetic profiling (DNA methylation) in regulating this polyphenism. Alternative morphs (N116 winged and N127 pale) were obtained through crowding experiment.","dates":{"release":"2026-04-10T00:00:00Z","modification":"2026-04-10T01:02:16.843Z","creation":"2025-06-09T12:32:54.407Z"},"accession":"E-MTAB-15212","cross_references":{"ENA":["ERP173279"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003750","EFO_0005518","EFO_0004184"]}}