biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsYim Zhe YangNextSeq 550RNA-seq of total RNAAcyrthosiphon pisumAcyrthosiphon pisumhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15213Different pea aphid response differently to stress response with certain genotype producing wing but others changing body colour morph, However, the underlying molecular mechanisms remains unclear. Therefore, we used RNA-seq to analyse the transcriptomic profile of the four different pea aphid morphs (N116 winged, N116 wingless, N127 red, N127 pale) to provide insights into the epigenetic profiling (DNA methylation) in regulating this polyphenism. Alternative morphs (N116 winged and N127 pale) were obtained through crowding experiment.biostudies-arrayexpressSample Collection - Samples were collected from the crowding experiment that is repeat 7 times.Nucleic Acid Extraction - Each biological replicate represent seven adult aphid (pooled samples of 1 aphid from each crowding experiment). Total RNA were extracted using the Qiagen RNAeasy Blood and Tissue KitSequencing - Library is sequenced on the Illumina HiSeq4000 instrument (76 + 76 cycle) and output data was demultiplex (allowing one mistmatch) and BCL to Fastq conversion were performed using illumina bcl2fastq software version 2.20.0.44Library Construction - ibrary were constructed using the TruSeq® Stranded mRNA assay (illumina)OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesYim Zhe YangfalseRNA-seq for 4 different pea aphid morphs to understanding polyphenismDifferent pea aphid response differently to stress response with certain genotype producing wing but others changing body colour morph, However, the underlying molecular mechanisms remains unclear. Therefore, we used RNA-seq to analyse the transcriptomic profile of the four different pea aphid morphs (N116 winged, N116 wingless, N127 red, N127 pale) to provide insights into the epigenetic profiling (DNA methylation) in regulating this polyphenism. Alternative morphs (N116 winged and N127 pale) were obtained through crowding experiment.2026-04-10T00:00:00Z2026-04-10T01:02:16.886Z2025-06-10T22:01:04.92ZE-MTAB-15213ERP173324EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184