{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics"],"submitter":["Hyosung Kim"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15239"],"description":["C3 sensor-equipped astrocytes activated by inflammatory cytokines"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - RNA was extracted using a Direct-zol RNA miniprep kit (Zymo R2050) according to the manufacturer’s instruction. This process was repeated two additional times from independent iPSC differentiations (biological n=3).","Sample Collection - Astrocytes derived from iPSCs were detached from MatriGel coated plate with Accutase. Cells were then rinsed with PBS and incubated in Trizol (Thermo Fisher 15596026) for 10 minutes at room temperature.","Library Construction - Raw sequencing reads were obtained for the 9paired-end samples.FASTQ reads were mapped to human genome (hg19) by HISAT2 (version 2.1.0)","Sequencing - RNA sequencing was performed by the Vanderbilt Technologies for Advanced Genomics (VANTAGE) facility using an Illumina NovaSeq 6000 instrument."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Ethan Lippmann","Hyosung Kim"],"additional_accession":[]},"is_claimable":false,"name":"Precision targeting of C3+ reactive astrocyte subpopulation with endogenous ADAR in iPSC-derived model","description":"C3 sensor-equipped astrocytes activated by inflammatory cytokines","dates":{"release":"2025-06-12T00:00:00Z","modification":"2025-06-18T15:24:49.583Z","creation":"2025-06-18T15:24:49.583Z"},"accession":"E-MTAB-15239","cross_references":{"ENA":["ERP173616"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}