{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Marek Marzec"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Hordeum vulgare"],"species":["Hordeum vulgare"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15240"],"description":["Identification of strigolactone-dependent genes in barley involved in response to cadmium (Cd) and zinc (Zn) treatment. Comparison of two organs (shoot and root) allowed to identification of tissue-specific genes that remain under the control of strigolactones in barley"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Samples were frozen immediately in liquid nitrogen; RNA was isolated using the mirVana miRNA Isolation Kit (Sigma, catalogue number: AM1560).","Sequencing - Transcriptome sequencing was conducted by Novogene Co., LTD (Beijing, China).","Sample Collection - For RNA-isolation analyses, plant tissue (shoot and root) was collected in four biological replicates, each containing tissue from 4 seedlings.","Growth Protocol - For the RNAseq experiment, plants were grown in hydroponic conditions up to 21 days. Six plants were placed in the 1.5 l container filled with ½ Hoagland solution (Hoagland and Dennis, 1938). The medium was replaced every week. Plants were placed in the greenhouse under a 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity. After eleven days of growth in control conditions, the containers were divided into variants: control, treated with Cd at a concentration of 5 µM, or treated with Zn at a concentration of 50 µM. The treatment lasted another ten days.","Library Construction - Preparation of RNA library was conducted by Novogene Co., LTD (Beijing, China)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Marek Marzec"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq; comparison of root and shoot transcriptome between WT and strigolactone-insensitive mutant (hvd14.d), barley in response to cadmium (Cd) and zinc (Zn) treatment; three-week-old seedlings","description":"Identification of strigolactone-dependent genes in barley involved in response to cadmium (Cd) and zinc (Zn) treatment. Comparison of two organs (shoot and root) allowed to identification of tissue-specific genes that remain under the control of strigolactones in barley","dates":{"release":"2026-05-31T00:00:00Z","modification":"2026-05-31T01:01:14.056Z","creation":"2025-06-12T13:40:19.987Z"},"accession":"E-MTAB-15240","cross_references":{"ENA":["ERP173420"],"Biostudies":["E-MTAB-13641"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003738","EFO_0004184"]}}