biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMarek MarzecIllumina NovaSeq 6000RNA-seq of coding RNAHordeum vulgareHordeum vulgarehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15240Identification of strigolactone-dependent genes in barley involved in response to cadmium (Cd) and zinc (Zn) treatment. Comparison of two organs (shoot and root) allowed to identification of tissue-specific genes that remain under the control of strigolactones in barleybiostudies-arrayexpressNucleic Acid Extraction - Samples were frozen immediately in liquid nitrogen; RNA was isolated using the mirVana miRNA Isolation Kit (Sigma, catalogue number: AM1560).Sequencing - Transcriptome sequencing was conducted by Novogene Co., LTD (Beijing, China).Sample Collection - For RNA-isolation analyses, plant tissue (shoot and root) was collected in four biological replicates, each containing tissue from 4 seedlings.Growth Protocol - For the RNAseq experiment, plants were grown in hydroponic conditions up to 21 days. Six plants were placed in the 1.5 l container filled with ½ Hoagland solution (Hoagland and Dennis, 1938). The medium was replaced every week. Plants were placed in the greenhouse under a 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity. After eleven days of growth in control conditions, the containers were divided into variants: control, treated with Cd at a concentration of 5 µM, or treated with Zn at a concentration of 50 µM. The treatment lasted another ten days.Library Construction - Preparation of RNA library was conducted by Novogene Co., LTD (Beijing, China).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMarek MarzecfalseRNA-seq; comparison of root and shoot transcriptome between WT and strigolactone-insensitive mutant (hvd14.d), barley in response to cadmium (Cd) and zinc (Zn) treatment; three-week-old seedlingsIdentification of strigolactone-dependent genes in barley involved in response to cadmium (Cd) and zinc (Zn) treatment. Comparison of two organs (shoot and root) allowed to identification of tissue-specific genes that remain under the control of strigolactones in barley2026-05-31T00:00:00Z2026-05-31T01:01:14.056Z2025-06-12T13:40:19.987ZE-MTAB-15240ERP173420E-MTAB-13641EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184